Two color flow cytometric analysis of FoxP1 expression in mouse thymocytes. Mouse thymocytes were stained with PerCP-Cy™5.5 Hamster Anti-Mouse CD3e antibody (Cat No. 561108)) and fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with either PE Mouse IgG2a, κ Isotype Control (Cat No. 554648, Left Panel) or PE Mouse Anti-FoxP1 antibody (Cat No. 564216; Right Panel). The flow cytometric dot plots show the correlated expression patterns of CD3 versus FoxP1 (or IgG2a Isotype Control staining) for gated events with the forward and side light-scatter characteristics of intact mouse thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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Two color flow cytometric analysis of FoxP1 expression in mouse thymocytes. Mouse thymocytes were stained with PerCP-Cy™5.5 Hamster Anti-Mouse CD3e antibody (Cat No. 561108)) and fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with either PE Mouse IgG2a, κ Isotype Control (Cat No. 554648, Left Panel) or PE Mouse Anti-FoxP1 antibody (Cat No. 564216; Right Panel). The flow cytometric dot plots show the correlated expression patterns of CD3 versus FoxP1 (or IgG2a Isotype Control staining) for gated events with the forward and side light-scatter characteristics of intact mouse thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
Forkhead box protein P1; hFKH1B; HSPC215; QRF1; 12CC4
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG2a, κ
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2738675
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures
PE Mouse anti-FoxP1 antibody stained normal human peripheral blood B cells and T cells with a low signal-to-noise ratio (S/N ¡ 2) upon flow cytometric analyses. It is therefore not recommended for staining normal peripheral blood leucocytes for flow cytometric analysis.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Transcription Factor Buffer Set RUO
Size
100 Tests
Cat No.
562574
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554648
PerCP-Cy™5.5 Hamster Anti-Mouse CD3e RUO
Size
25 µg
Cat No.
561108
Transcription Factor Buffer Set RUO
Size
25 Tests
Cat No.
562725
564216 Rev. 2
Antibody Details
JC12
The JC12 monoclonal antibody specifically binds to Forkhead box protein P1 (FoxP1). FoxP1 belongs to subfamily P of the forkhead box (FOX) winged-helix transcription factor family. It plays a variety of roles such as regulating the formation of lymphoid, lung, brain and heart tissues. FoxP1 is critical in regulating early B cell development and is also required for maintenance of naïve T cell quiescence, and monocyte differentiation and macrophage function. Aberrant expression of FOXP1 has been linked with mucosa-associated lymphoid tissue lymphoma and diffuse-large B cell lymphoma.
564216 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564216 Rev.2
Citations & References
Development References (10)
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Banham AH, Beasley N, Campo E, et al. The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p. Cancer Res. 2001; 61(24):8820-8829. (Immunogen: Immunohistochemistry, Western blot). View Reference
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Brown PJ, Ashe SL, Leich E, et al. Potentially oncogenic B-cell activation-induced smaller isoforms of FOXP1 are highly expressed in the activated B cell-like subtype of DLBCL. Blood. 2008; 111(5):2816-2824. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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Feng X, Wang H, Takata H, Day TJ, Willen J, Hu H. Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells. Nat Immunol. 2011; 12(6):544-550. (Biology). View Reference
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Hu H, Wang B, Borde M, et al. Foxp1 is an essential transcriptional regulator of B cell development. Nat Immunol. 2006; 7(8):819-826. (Biology). View Reference
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Katoh M, Igarashi M, Fukuda H, Nakagama H, Katoh M. Cancer genetics and genomics of human FOX family genes. Cancer Lett. 2013; 328(2):198-206. (Biology). View Reference
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Sagardoy A, Martinez-Ferrandis JI, Roa S, et al. Downregulation of FOXP1 is required during germinal center B-cell function. Blood. 2013; 121(21):4311-4320. (Biology). View Reference
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Shi C, Sakuma M, Mooroka T, et al. Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function. Blood. 2008; 112(12):4699-4711. (Biology). View Reference
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Streubel B, Vinatzer U, Lamprecht A, Raderer M, Chott A. T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma. Leukemia. 2005; 19(4):652-658. (Biology). View Reference
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Wang B, Lin D, Li C, Tucker P. Multiple domains define the expression and regulatory properties of Foxp1 forkhead transcriptional repressor. J Biol Chem. 2003; 278(27):24259-24268. (Biology). View Reference
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Wlodarska I, Veyt E, De Paepe P, et al. FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations. Leukemia. 2005; 19(8):1299-1305. (Clone-specific: Immunohistochemistry). View Reference
564216 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
