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Western blot analysis of KIF1A expression on rat embryonic (E21) cerebrum lysate. Rat cerebrum lysate (Cat. No. 611463) was stained with Purified Mouse Anti-KIF1A (Cat. No. 612094) at a 1:500 (Lane 1), 1:1000 (Lane 2), and 1:2000 (Lane 3) dilution. KIF1A expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).
BD Transduction Laboratories™ Purified Mouse Anti-KIF1A
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: For additional technical guidance, please refer to the protocols under "Cell Biology (WB, IP, IHC, IF)" at our website: http://www.bdbiosciences.com/us/s/resources.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The ability of the kinesin superfamily of motor proteins to hydrolyze ATP as they move progressively along microtubules is important for organelle transport and cell division. Kinesins are grouped according to the location of the motor domain in the N-terminal, middle, or C-terminal region of the protein. A family of N-terminal motor domain kinesin proteins includes KIF1A/1B, KIF3A/3B, KRP85/95, and Klp68d/64. KIF1A and KIF1B are 93% homologous in their N-terminal motor domains, however KIF1A contains a C-terminal PH domain. KIF1A and KIF1B are expressed in neurons where KIF1A is involved in fast anterograde axon transport of synaptic vesicles, and KIF1B is involved in anterograde axon transport of mitochondria. KIF1A associates with organelles that contain synaptotagmin, synaptophysin, and Rab3A. Disruption of the KIF1A gene in mice causes decreases in synaptic vesicle density, neuronal degeneration, and deficits in motor and sensory function. Thus, KIF1A is a critical brain motor protein involved with axonal transport of synaptic vesicle precursors.
Development References (4)
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Bloom GS. The UNC-104/KIF1 family of kinesins. Curr Opin Cell Biol. 2001; 13(1):36-40. (Biology). View Reference
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Chung CY, Koprich JB, Siddiqi H, Isacson O.. Dynamic changes in presynaptic and axonal transport proteins combined with striatal neuroinflammation precede dopaminergic neuronal loss in a rat model of AAV alpha-synucleinopathy. J Neurosci. 2009; 29(11):3365-3373. (Biology). View Reference
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Okada Y, Yamazaki H, Sekine-Aizawa Y, Hirokawa N. The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors. Cell. 1995; 81(5):769-780. (Biology). View Reference
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Yonekawa Y, Harada A, Okada Y. Defect in synaptic vesicle precursor transport and neuronal cell death in KIF1A motor protein-deficient mice. J Cell Biol. 1998; 141(2):431-441. (Biology). View Reference
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