BD Pharmingen™ Purified Mouse Anti-Human IKKγ
Clone C73-1794 (RUO)
Western blot analysis of IKKγ in SK-N-SH human neuroblastoma cell lysates. Lane 1. anti-IKKγ. Lane 2, a mouse IgG1 isotope control.
Western blot analysis of IKKγ in SK-N-SH human neuroblastoma cell lysates. Lane 1. anti-IKKγ. Lane 2, a mouse IgG1 isotope control.
Preparation And Storage
Recommended Assay Procedures
The C73-1794 antibody may be used for western blot analysis (2 µg/ml). Jurkat human T cells (ATCC TIB-152) and SK-N-NH human neuroblastoma cells (ATCC HTB-11) are suggested as positive controls for this application.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The nuclear transcription factor kappa-B, NF-κB, is controlled by interaction with an inhibitory subunit, I-κB, which restricts NF-κB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-κB becomes degraded and NF- κB is released to the nucleus. The release of I-κB from NF-κB is thought to be a critical point in the activation of NF-κB signal pathways. A group of proteins form an NF-κB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-κB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKα, IKKβ as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ. IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK)4 and MEKK1. IKKγ interacts preferentially with IKKβ and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-κB. IKKγ forms migrate as a doublet of ~50-55 kDa in SDS-PAGE. Clone C73-1794 recognizes human IKKγ. Purified, recombinant human IKKγ was used as immunogen.
Development References (5)
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DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 1997; 388(6642):548-554. (Biology). View Reference
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Ling L, Cao Z, Goeddel DV. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. 1998; 95(7):3792-3797. (Biology). View Reference
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Mercurio F, Zhu H, Murray BW, et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science. 1997; 278(5339):860-866. (Biology). View Reference
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Nakano H, Shindo M, Sakon S, et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. 1998; 95(7):3537-3542. (Biology). View Reference
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Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Clone-specific: Activation). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.