BD Pharmingen™ Purified Mouse Anti-Human Cyclin F

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes.  Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. Most cyclins also contain a so-called "destruction box" motif, which targets cyclins for rapid, ubiquitin-mediated degradation, as well as PEST sequences, which are thought to result in protein instability. Certain cyclins may have additional functions not restricted to cell cycle regulation. Thus, cyclins have been placed into functional groups as follows:  Group 1 (cyclins A, B, D1, D2, D3, E and F) functions primarily in cell cycle reguation; Group 2 (cyclins C and H) may also play a role in transcriptional regulation; Group 3 (cyclins G1, G2 and I) may play a role distinct from either cell cycle or transcriptional regulation. Cyclin F is a novel cyclin which is structurally most similar to cyclin A, yet cyclin F lacks the destruction box sequence.  Cyclin F is the largest of the cyclins, with a predicted M.W. of 87 kD and an observed M.W. of 100-110 kD, which is thought to reflect posttranslational modification of the protein.  Like cyclin A, expression of cyclin F is low or undetectable in G0, begins to accumulate in Sphase, peaks in G2 and decreases at M-phase. Overexpression of cyclin F results in an accumulation of cells in G2, suggesting that this cyclin plays a role in G2/M transition during the cell cycle. B74-2 reacts with human cyclin F. A polypeptide fragment containing amino acids 693-776 of immunogen.