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      Purified Mouse anti-Ezrin (pY353)
      558033_image1.png

      Western blot analysis of Ezrin (pY353). Human K562 cells were either left untreated (lanes 1-3) or treated with pervanadate (lanes 4-6). Blots were probed with anti-Ezrin (pY353) antibody at concentrations of 0.125 (lanes 1 and 4), 0.063 (lanes 2 and 5), and 0.032 ug/ml (lanes 3 and 6). Ezrin (pY353) is identified as a band of ~80 kDa

      558033_image1.png Blue-Cap-White-Label.jpg

      Western blot analysis of Ezrin (pY353). Human K562 cells were either left untreated (lanes 1-3) or treated with pervanadate (lanes 4-6). Blots were probed with anti-Ezrin (pY353) antibody at concentrations of 0.125 (lanes 1 and 4), 0.063 (lanes 2 and 5), and 0.032 ug/ml (lanes 3 and 6). Ezrin (pY353) is identified as a band of ~80 kDa

      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      Phosphorylated Human Ezrin Peptide
      Western blot (Routinely Tested)
      80 kDa
      0.25 mg/ml
      AB_396998
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      558033 Rev. 3
      Antibody Details
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      I66-386

      Ezrin is a member of the ERM (ezrin-radixin-moesin) family of proteins that function as crosslinkers between the actin cytoskeleton and the plasma membrane.  Ezrin is a substrate for the tyrosine kinase epidermal (EGF) and hepatocyte growth factor (HGF) receptors.  Upon EGF or HGF stimulation, ezrin is phosphorylated on tyrosine 353 (Y353), which is required for its interaction with phosphatidylinositol 3 kinase p85 subunit and activation of Akt.

      The I66-386 monoclonal antibody recognizes the phosphorylated Y353 of human ezrin.

      558033 Rev. 3
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558033 Rev.3
      Citations & References
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      View product citations for antibody "558033" on CiteAb

      Development References (1)

      1. Gautreau A, Poullet P, Louvard D, Arpin M. Ezrin, a plasma membrane–microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999; 96:7300-7305. (Biology). View Reference
      558033 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.