BD Pharmingen™ Serum Rabbit Anti-Cdk2

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell cycle control in eukaryotes. Cdks are catalytic subunits whose activity requires interaction with their regulatory subunits, the cyclins, as well as specific phosphorylation events. The precise timing of cyclin-cdk activity during the cell cycle determines whether the cell cycle continues or becomes blocked. Cdks 2, 4, 5, and 6 may associate with D-type cyclins. Interaction between cdk2 and cyclin E during G1/S transition creates a complex with histone H1 kinase activity. This complex is thought to be required for the initiation and progression of DNA replication during S phase. Cdk2-cyclin A complexes appear during late S phase and also play a role in progression of DNA replication. Substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes, (c-src, c-abl, SV40 large-T), tumor suppressor genes (e.g., RB and p53), nucleolin, RNA polymerase II and others.  A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen to generate this antibody.

The polyclonal antibodies recognize human and mouse cdk2. The antibodies do not react with other known cdk proteins. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen.