Product Details
BD Transduction Laboratories™
Human (QC Testing), Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human VHR aa. 1-185
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
20 kDa
250 µg/ml
AB_397904
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610547 Rev. 1
Antibody Details
24/VHR
Protein tyrosine phosphatases (PTP) have been implicated in cell proliferation, cell signaling, and oncogenic transformation. More members of the PTP family of proteins have been found by screening libraries with cDNA at low stringency hybridization. VHR, a prototype for PTP, is a vaccinia-virus (VH-1)-related phosphatase of 185 amino acids with activity against phosphotyrosine and phosphoserine. In vitro, VHR is capable of dephosphorylating several growth factor receptors. In conjunction with insulin or progesterone, VHR accelerates germinal vesicle breakdown of Xenopus oocytes. These findings suggest that VHR is implicated in the regulation of cyclin-dependent kinases during the cell cycle.
610547 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610547 Rev.1
Citations & References
View product citations for antibody "610547" on CiteAb
Development References (4)
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Alonso A, Saxena M, Williams S, Mustelin T. Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activation. J Biol Chem. 2001; 276(7):4766-4771. (Clone-specific: Western blot). View Reference
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Aroca P, Bottaro DP, Ishibashi T, Aaronson SA, Santos E. Human dual specificity phosphatase VHR activates maturation promotion factor and triggers meiotic maturation in Xenopus oocytes. J Biol Chem. 1995; 270(23):14229-14234. (Biology). View Reference
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Ishibashi T, Bottaro DP, Chan A, Miki T, Aaronson SA. Expression cloning of a human dual-specificity phosphatase. Proc Natl Acad Sci U S A. 1992; 89(24):12170-12174. (Biology). View Reference
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Yuvaniyama J, Denu JM, Dixon JE, Saper MA. Crystal structure of the dual specificity protein phosphatase VHR. Science. 1996; 272(5266):1328-1331. (Biology). View Reference
610547 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.