-
Your selected country is
Singapore
- Change country/language
-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
- Immunoassay Reagents
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ TCR/BCR Profiling Assays for Human and Mouse
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
-
Functional Assays
-
Microscopy and Imaging Reagents
-
Cell Preparation and Separation Reagents
-
Training
- Flow Cytometry Basic Training
-
Product-Based Training
- FACSAria Product Based Training
- FACSMelody Product-Based Training
- FACSLyric Product-Based Training
- FACSCanto Product-Based Training
- LSRFortessa Product-Based Training
- FACSymphony Product-Based Training
- FACSDuet Product-Based Training
- HTS Product-Based Training
- BD FACSDiscover™ S8 Cell Sorter Product Training
-
Advanced Training
-
Thought Leadership
-
Product News
- Blogs
- Scientific Publications
-
Events
- Nature Research Academies Workshop 2023
- CYTO 2023: Advancing the World of Cytometry
- Singapore Gene & Cell Therapy Conference 2023
- EuroFlow Educational Workshop
- Nature Research Masterclass 2023
- Novel Approaches to Single-Cell Plant Research: from Real-Time Imaging Cell Sorting to Single-Nuclei Transcriptomics
- Advances in Immune Monitoring Series
-
Product News
-
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ TCR/BCR Profiling Assays for Human and Mouse
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
-
- FACSAria Product Based Training
- FACSMelody Product-Based Training
- FACSLyric Product-Based Training
- FACSCanto Product-Based Training
- LSRFortessa Product-Based Training
- FACSymphony Product-Based Training
- FACSDuet Product-Based Training
- HTS Product-Based Training
- BD FACSDiscover™ S8 Cell Sorter Product Training
-
- Nature Research Academies Workshop 2023
- CYTO 2023: Advancing the World of Cytometry
- Singapore Gene & Cell Therapy Conference 2023
- EuroFlow Educational Workshop
- Nature Research Masterclass 2023
- Novel Approaches to Single-Cell Plant Research: from Real-Time Imaging Cell Sorting to Single-Nuclei Transcriptomics
- Advances in Immune Monitoring Series
- Singapore (English)
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Western blot analysis of PMS2. Lysate from A431 human epidermal cells was probed with anti-PMS2 (clone A16-4, Cat. No. 556415) between 2.0 and 0.08 µg/ml and identifies PMS2 at ~100 kDa.
BD Pharmingen™ Purified Mouse Anti-PMS2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In humans the proteins are called MutS homolog2 (MSH2), MutL homolog (MLH1), and PMS2 which is also a homolog of MutL. After MSH2 and a partner bind to a mismatched DNA duplex, the complex is joined by a heterodimer of MLH1 and PMS2 which together help facilitate the later steps in mismatch repair. Two other members of this family, MSH3 and MSH6, can also join the MSH2-containing complex to help facilitate repair. The reduced molecular weight of PMS2 is ~100 kDa. mAb A16-4 recognizes human and mouse PMS2. Recombinant human PMS2 (C-terminal half) was used as immunogen.
Development References (5)
-
Cleaver JE. It was a very good year for DNA repair. Cell. 1994; 76(1):1-4. (Biology). View Reference
-
Marsischky GT, Filosi N, Kane MF, Kolodner R. Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair. Genes Dev. 1996; 10(4):407-420. (Biology). View Reference
-
Prolla TA, Christie DM, Liskay RM. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Mol Cell Biol. 1994; 14(1):407-415. (Biology). View Reference
-
Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994; 265(5175):1091-1093. (Biology). View Reference
-
Su SS, Modrich P. Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc Natl Acad Sci U S A. 1986; 83(14):5057-5061. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.