Product Details
Description
Human eotaxin is a 74-amino acid protein with molecular weight of 8.4 kDa. Eotaxin belongs to the C-C chemokine family and is a potent and selective chemoattractant for eosinophils, but not monocytes, lymphocytes or neutrophils. C-C chemokine receptor 3 (CCR3) has been identified as the sole receptor for human eotaxin. Recombinant human eotaxin (Cat. No. 555102) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives. Recombinant human eotaxin is ≥ 95% pure as measured by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human eotaxin protein, as measured in a chromogenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Upon initial thawing, recombinant human eotaxin (Cat. No. 555102) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 1.0 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. Carrier proteins should be pre-screened for possible effects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Recombinant human eotaxin (Cat. No. 555102) is useful as a quantitative standard for measuring human eotaxin protein levels using sandwich ELISA with Purified Mouse Anti-Human Eotaxin antibody clone 3C7 (Cat. No. 555035) as a capture antibody and Biotin Mouse Anti-Human Eotaxin antibody clone 10C11 (Cat. No. 555060) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant human eotaxin from 1,000 - 20 pg/mL to be included in each ELISA plate. For measuring human eotaxin in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human Eotaxin Set (Cat. No. 555175).
Bioassay: Recombinant human eotaxin (Cat. No. 555102) has been reported to possess chemotactic activity for human eosinophils in chemotaxis assays. Investigators are advised that the bioassay application is not routinely tested for this material and are highly encouraged to both titrate and include appropriate controls in relevant experiments.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.