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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form).
This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines: Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- γ, MCP-1, MCP-2, MCP-3, MIP-1α, MIP-1β, NAP-2, PF4, RANTES, SCF, TNF, LT-α.
Neutralization: Investigators are advised that this material is not routinely tested for the Neutralization application and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. This antibody has previously been reported to be useful for the neutralization of recombinant human IL-8 when measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells. Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization with the following representative ranges:
50% Neutralization (ND50) at 8 - 10 μg/mL
> 95% Neutralization at 150 - 500 μg/mL
Development References (2)
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Leonard EJ, Sylvester I, Yoshimura T, et al. Current Protocols in Immunology. John Wiley and Sons; 1995:6.12.1-6.12.26.
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Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Clone-specific). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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