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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
ELISA: Purified Mouse Anti-Human IFN-γ antibody (Clone NIB42, Cat. No 551221) can be useful as a capture antibody in sandwich ELISA for measuring human IFN-γ protein levels. Purified NIB42 antibody can be paired with Biotin Mouse Anti-Human IFN-γ mAb, (Clone 4S.B3, Cat. No. 554550) as the detection antibody along with recombinant human IFN-γ (Cat. No. 554617) as the standard. For measuring human IFN-γ in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IFN-γ ELISA Set (Cat. No. 555142) or BD OptEIA™ Human IFN-γ ELISA Kit II (Cat. No. 550612).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The NIB42 antibody reacts with human interferon-gamma (IFN-γ). The immunogen used to generate the NIB42 hybridoma was recombinant human IFN-γ. This is a neutralizing antibody.
Development References (2)
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Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology). View Reference
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Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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