BD Pharmingen™ Biotin Mouse Anti-Human IL-9
Clone MH9A3 (RUO)
Curve from a sandwich ELISA that measures IL-9 protein levels, generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody. Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm. The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).
Curve from a sandwich ELISA that measures IL-9 protein levels, generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody. Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm. The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).
Product Details
Description
The MH9A3 monoclonal antibody specifically binds to human interleukin-9 (IL-9). Human IL-9 is a multifunctional cytokine and a member of the type I cytokine (hematopoietin) family that includes IL-2, IL-4, IL-7, IL-15 and IL-21. This cytokine is encoded by the IL9 gene that is resident on chromosome 5q31.1. IL-9 is expressed by activated CD4-positive T helper cells, by some transformed T cells and by eosinophils, mast cells and neutrophils. IL-9 induces the proliferation, differentiation, and effector function of various cell types including T lymphocytes, B lymphocytes, mast cells, eosinophils, neutrophils, hematopoietic cells and epithelial cells. It potentiates the interleukin-4-induced IgM, IgG and IgE responses by human B lymphocytes. IL-9 has been implicated in human allergic disorders such as asthma and malignancies such as Hodgkin's disease. IL-9 exerts its biological activities through binding to the surface IL-9 receptor (IL-9R) complex comprised of the IL-9R alpha subunit (IL-9Rα; CD129) and the common cytokine receptor gamma subunit (γc; CD132). IL-9 signaling through its receptor includes activation of the Janus kinases 1 and 3 ( JAK1 and JAK3) and activation of Signal transducer and activator of transcription 1, 3 and 5 factors (STAT1, STAT3 and STAT5).
Preparation And Storage
Recommended Assay Procedures
The biotinylated MH9A3 antibody is useful as a detection antibody for a sandwich ELISA that measures human IL-9 protein levels. The biotinylated MH9A3 antibody can be paired with the purified MH9A4 anti-human IL-9 (Cat. No. 558088) as the capture antibody, with recombinant human IL-9 protein as the standard. The biotinylated MH9A3 antibody should be titrated between 0.25 - 2 µg/ml to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of recombinant human IL-9 protein ranging from 1.0 to 125 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology information, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis application Handbook 1st Edition. BD Biosciences. Available for download as the Immune Function Application handbook from
http://www.bdbiosciences.com/cgi-bin/literature/archive?type=2&brand=0
Note: This ELISA antibody pair shows no crossreactivity with the following recombinant human cytokines: IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-18, IFNγ, gp130, TNF, 4-1BB. This ELISA antibody pair also shows no cross-reactivity with recombinant cytokines from other species: mouse IL-9.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.