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Purified Mouse Anti-Human Ig κ Light Chain
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b, κ
ELISA (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

This antibody can be used to detect human immunoglobulin ligh chain kappa (κ). In a quantitative sandwich ELISA for measuring immunoglobulin light chain kappa, this antibody may be paired with purified anti-human immunoglobulin, clone G18-145 (Cat. No. 555785).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555861 Rev. 6
Antibody Details
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The G20-361 antibody reacts with human immunoglobulin light chain kappa (κ). It does not react with λ light chain or heavy chains.

This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555861 Rev. 6
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555861 Rev.6
Citations & References
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555861 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.