Skip to main content Skip to navigation
Purified Rat Anti-Human CD49f
Purified Rat Anti-Human CD49f

Flow cytometric analysis of CD49f expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841; dashed line histogram) or Purified Rat Anti-Human CD49f (Cat. No. 555734; solid line histogram), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms depicting CD49f (or Ig isotype) expression were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD49f expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841; dashed line histogram) or Purified Rat Anti-Human CD49f (Cat. No. 555734; solid line histogram), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms depicting CD49f (or Ig isotype) expression were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
ITGA6; IThe GoH3 monoclonal ntegrin alpha-6; Integrin α6 chain; VLA-6; ITA6
Human (QC Testing), Mouse, Pig, Dog (Tested in Development)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Mouse mammary tumor cells
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Reported)
0.5 mg/ml
IV P55
3655
AB_2296273
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555734 Rev. 6
Antibody Details
Down Arrow Up Arrow
GoH3

The GoH3 monoclonal antibody specifically binds to CD49f which is also known as integrin α6 chain. CD49f is a ~150 kDa type I transmembrane glycoprotein that belongs to the integrin alpha chain family of extracellular matrix and cell adhesion receptors. The integrin α6 subunit associates with the integrin β1 chain (CD29) to form VLA-6 and with the integrin β4 chain (CD104) to form the integrin α6β4 complex, also known as the laminin and kalinin receptor. CD49f is expressed mainly on T cells, monocytes, platelets, epithelial cells, endothelial cells, perineural cells, and trophoblasts of placenta. GoH3 recognizes an extracellular epitope of integrin α6 on human, mouse and bovine cells. GoH3 has been reported to block the binding of integrin α6 to laminin P1 and E8 fragments.

555734 Rev. 6
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555734 Rev.6
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp Cell Res. 1990; 188(1):55-60. (Biology). View Reference
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J. Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. J Histochem Cytochem. 1986; 34(8):1037-1046. (Immunogen). View Reference
555734 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.