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PE Rat Anti-Mouse CD41
PE Rat Anti-Mouse CD41

Flow cytometric analysis of CD41 expression on mouse platelets.  Mouse platelets were stained with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; dashed line histogram) or PE Rat Anti-Mouse CD41 antibody (Cat. No. 558040/561850; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets.

Flow cytometric analysis of CD41 expression on mouse platelets.  Mouse platelets were stained with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; dashed line histogram) or PE Rat Anti-Mouse CD41 antibody (Cat. No. 558040/561850; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets.

Product Details
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BD Pharmingen™
Itga2b; Integrin alpha 2b; Integrin alpha-IIb; Integrin α IIb; GpIIb
Mouse (QC Testing)
Rat IgG1, κ
Mouse Platelets
Flow cytometry (Routinely Tested)
0.2 mg/ml
16399
AB_397004
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558040 Rev. 4
Antibody Details
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MWReg30

The MWReg30 monoclonal antibody specifically binds to CD41, which is also known as glycoprotein IIb (gpIIb, GP IIb), Integrin α IIb chain, or Integrin alpha 2b. CD41 is a transmembrane glycoprotein that is encoded by Itga2b. CD41 associates with Integrin β3 chain (gpIIIa or CD61) to form the gpIIb/IIIa (CD41/CD61) complex. CD41/CD61 is expressed on platelets, megakaryocytes, and early hematopoietic progenitors. The integrin complex binds to fibrinogen, fibronectin, vitronectin, von Willebrand factor, and thrombospondin. It is important for platelet adhesion and aggregation, and it may play a role in osteolytic tumor metastasis.

558040 Rev. 4
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558040 Rev.4
Citations & References
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Development References (5)

  1. Bakewell SJ, Nestor P, Prasad S, et al. Platelet and osteoclast beta3 integrins are critical for bone metastasis. Proc Natl Acad Sci U S A. 2003; 100(24):14205-14210. (Biology). View Reference
  2. Ferkowicz MJ, Starr M, Xie X, et al. CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003; 130(18):4393-4403. (Biology). View Reference
  3. Kieffer N, Phillips DR. Platelet membrane glycoproteins: functions in cellular interactions. Annu Rev Cell Biol. 1990; 6:329-357. (Biology). View Reference
  4. Nieswandt B, Echtenacher B, Wachs FP, et al. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood. 1999; 94(2):684-693. (Immunogen). View Reference
  5. Phillips DR, Charo IF, Scarborough RM. GPIIb-IIIa: the responsive integrin. Cell. 1991; 65(3):359-362. (Biology). View Reference
View All (5) View Less
558040 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.