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PE Mouse Anti-Human TACTILE (CD96)
PE Mouse Anti-Human TACTILE (CD96)
Two-color flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus CD3 for gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
PE Mouse Anti-Human TACTILE (CD96)
Multiparameter flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat No. 561811) and either PE Mouse IgG1, κ Isotype Control (Cat No. 554680; Left Plot) or PE Mouse Anti-Human TACTILE (CD96) antibody (Cat No. 568271/568272; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Two-color flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus CD3 for gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat No. 561811) and either PE Mouse IgG1, κ Isotype Control (Cat No. 554680; Left Plot) or PE Mouse Anti-Human TACTILE (CD96) antibody (Cat No. 568271/568272; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
hCD96; T cell-activated increased late expression protein
Human (QC Testing)
Mouse IgG1, κ
Human NK92 Cell Line
Flow cytometry (Routinely Tested)
5 µl
10225
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Antibody Details
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NK92.39.rMAb

The NK92.39.rMAb monoclonal antibody specifically recognizes human CD96 which is also known as TACTILE (T cell activation increased late expression). CD96 is a type I membrane glycoprotein comprised of three N-terminal Ig-like extracellular domains followed by one mucin-like domain, a transmembrane region, and a cytoplasmic tail with one basic/proline rich motif, a single ITIM motif, and an YXXM motif which is also present in ICOS and CD28. CD96 is expressed at low levels on resting natural killer (NK) cells and T cells and at high levels on activated NK and T cells. CD96 is also expressed at low levels on some B cells and on some T-cell leukemia and acute myeloid leukemia cells. CD96 plays a role in the adhesive interactions of activated NK and T cells with target cells during immune responses. CD96 binds to the poliovirus receptor (CD155) that is highly expressed on some tumor cells. CD155-mediated ligation of CD96 can activate NK cell-mediated cytotoxicity.

Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
Citations & References
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Development References (3)

  1. El-Sherbiny YM, Meade JL, Holmes TD, et al. The requirement for DNAM-1, NKG2D, and NKp46 in the natural killer cell-mediated killing of myeloma cells.. Cancer Res. 2007; 67(18):8444-9. (Clone-specific: Flow cytometry). View Reference
  2. Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).. J Immunol. 2004; 172(7):3994-8. (Immunogen: Blocking, Flow cytometry). View Reference
  3. Toutirais O, Cabillic F, Le Friec G, et al. DNAX accessory molecule-1 (CD226) promotes human hepatocellular carcinoma cell lysis by Vgamma9Vdelta2 T cells.. Eur J Immunol. 2009; 39(5):1361-8. (Clone-specific: Flow cytometry, Functional assay). View Reference

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.