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FITC Rat Anti-Mouse IL-12 (p40/p70)
FITC Rat Anti-Mouse IL-12 (p40/p70)
Flow cytometric analysis for IL-12 in activated mouse macrophages.  Mouse Intracellular Cytokine-3 positive control cells (MiCK-3) offered by BD Biosciences as MN 554654, are activated mouse macrophages prepared in the presence of a protein transport inhibitor.  Fixed and permeabilized MiCK-3 cells were stained either with a FITC Rat IgG1, κ isotype control (left panel) or with the FITC Rat Anti-Mouse IL-12 (p40/p70) antibody (right panel).  Dot plots were derived from gated events based on the light scattering characterisitcs for macrophages.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Flow cytometric analysis for IL-12 in activated mouse macrophages.  Mouse Intracellular Cytokine-3 positive control cells (MiCK-3) offered by BD Biosciences as MN 554654, are activated mouse macrophages prepared in the presence of a protein transport inhibitor.  Fixed and permeabilized MiCK-3 cells were stained either with a FITC Rat IgG1, κ isotype control (left panel) or with the FITC Rat Anti-Mouse IL-12 (p40/p70) antibody (right panel).  Dot plots were derived from gated events based on the light scattering characterisitcs for macrophages.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG1
CHO-expressed recombinant mouse IL-12 p70 protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
AB_1645243
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

Flow cytometry: The FITC Rat Anti-Mouse IL-12 (p40/p70) antibody can be used for the enumeration of IL-12 producing cells within mixed cell populations. A useful control investigators may consider using for demonstrating specificity of staining is to pre-block with one of the following reagents:  (1) recombinant mouse IL-12 (p40) (Cat. No. 554594) or with (2) unlabeled C15.6 antibody (Cat. No. 554477), prior to staining.  Intracellular staining techniques and the use of blocking controls are described by C. Prussin and D. Metcalfe.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560564 Rev. 1
Antibody Details
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C15.6

The C15.6 monoclonal antibody specifically binds to both free and complexed (homodimer p80 and heterodimer p70) forms of the p40 subunit of mouse interleukin-12 (IL-12). The immunogen used to generate the C15.6 hybridoma was recombinant mouse IL-12 p70 protein. p40 has also been described as a subunit of IL-23 and thus it is possible that the C15.6 antibody will crossreact with IL-23.

560564 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
560564 Rev.1
Citations & References
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View product citations for antibody "560564" on CiteAb

Development References (8)

  1. D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Biology). View Reference
  2. Gately MK, Chizzonite R, Presky DH. Measurement of Human and Mouse Interleukin-12. In: Cooligan J, Kruisbeek A, Margulies D, Shevach E, Storber W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:6-16.
  3. Gately MK, Renzetti LM, Magram J, et al. The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses. Annu Rev Immunol. 1998; 16:495-521. (Biology). View Reference
  4. Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). View Reference
  5. Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med. 1995; 182(5):1281-1290. (Biology). View Reference
  6. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). View Reference
  7. Wu C, Ferrante J, Gately MK, Magram J. Characterization of IL-12 receptor beta1 chain (IL-12Rbeta1)-deficient mice: IL-12Rbeta1 is an essential component of the functional mouse IL-12 receptor. J Immunol. 1997; 159(4):1658-1665. (Biology). View Reference
  8. Wysocka M, Kubin M, Vieira LQ, et al. Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur J Immunol. 1995; 25(3):672-676. (Biology: ELISA). View Reference
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560564 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.