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Product Details
BD OptiBuild™
IL17RA; IL17R; interleukin-17 receptor A; IL-17 receptor A; hIL-17R; CANDF5; IMD51
Human (Tested in Development)
Mouse IgG1, κ
Human IL-17RA Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2873916
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 605 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Companion Products
Human BD Fc Block™ RUO
Size
50 µg
Cat No.
564219
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
749614 Rev. 2
Antibody Details
W23-251
The W23-251 monoclonal antibody specifically recognizes IL-17RA (CD217), which is also known as Interleukin-17 receptor A. IL-17RA (CD217) is a ~120 kDa single-pass type I transmembrane glycoprotein that is encoded by IL17RA. IL-17RA (CD217) belongs to the IL-17 receptor family which also includes IL-17 RB, IL-17RC, IL-17RD, and IL-17RE. IL-17RA (CD217) has a wide tissue distribution and is variably expressed on granulocytes, monocytes, macrophages, dendritic cells (DCs), T cells, B cells, NK cells, smooth muscle cells, endothelial cells, and epithelial cells. Interleukin-17A (IL-17A) or IL-17F homodimers, or IL-17A:IL-17F heterodimers can bind to and signal through functional IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RC subunits. This leads to the cellular release of proinflammatory cytokines including IL-1, IL-6, and TNF, antimicrobial peptides, and chemokines that attract neutrophils. IL-17E/IL-25 binds to and signals through another functional IL-17 receptor complex comprised of IL-17RA (CD217) and IL-17RB subunits. IL-17E/IL-25 induces type-2 (Th2-like) immune responses and is implicated in asthma. IL-17C binds to IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RE subunits and can stimulate the production of proinflammatory cytokines and antimicrobial peptides.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
749614 Rev. 2
Format Details
BV605
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV605
407 nm
605 nm
749614 Rev.2
Citations & References
View product citations for antibody "749614" on CiteAb
Development References (3)
-
Gaffen SL. Structure and signalling in the IL-17 receptor family.. Nat Rev Immunol. 2009; 9(8):556-67. (Biology). View Reference
-
Toy D, Kugler D, Wolfson M, et al. Cutting edge: interleukin 17 signals through a heteromeric receptor complex.. J Immunol. 2006; 177(1):36-9. (Biology). View Reference
-
Yao Z, Spriggs MK, Derry JM, et al. Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine. 1997; 9(11):794-800. (Biology). View Reference
749614 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.