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BV421 Armenian Hamster Anti-Mouse Vγ1.1 TCR
BV421 Armenian Hamster Anti-Mouse Vγ1.1 TCR
Two-color flow cytometric analysis of Vγ1.1 TCR expression on mouse lymph node cells. C57BL/6 mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either BD Horizon™ PE-CF594 Hamster Anti-Mouse γδ T-Cell Receptor antibody alone (Cat. No. 563532; Left Plot), or simultaneously with BD Horizon™ BV421 Vγ1.1 TCR antibody (Cat. No. 566308; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of γδ T-Cell Receptor versus Vγ1.1 TCR (or Autofluorescence), were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
Two-color flow cytometric analysis of Vγ1.1 TCR expression on mouse lymph node cells. C57BL/6 mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either BD Horizon™ PE-CF594 Hamster Anti-Mouse γδ T-Cell Receptor antibody alone (Cat. No. 563532; Left Plot), or simultaneously with BD Horizon™ BV421 Vγ1.1 TCR antibody (Cat. No. 566308; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of γδ T-Cell Receptor versus Vγ1.1 TCR (or Autofluorescence), were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
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BD Horizon™
TCR V gamma 1.1; TCR Vg1.1; TCR V-gamma-1; TCR Vg1; Cr4
Mouse (QC Testing)
Armenian Hamster IgG
Mouse TCR γδ+ T3.13.1 hybridoma cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
107692
AB_2739676
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. CF™ is a trademark of Biotium, Inc.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  10. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  11. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566308 Rev. 1
Antibody Details
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2.11

The 2.11 monoclonal antibody recognizes the Vγ1.1 chain of the heterodimeric γδ T-cell receptor (TCR γδ) expressed by T lymphocytes and NK-T cells. It does not react with TCR αβ-bearing T cells. TCR γδ associates with CD3 molecules to form the TCR γδ/CD3 complex that mediates antigen recognition and can transduce intracellular signals leading to T cell responses. The Vγ1.1 TCR is also known as Vγ1 TCR. The Vγ1.1 variable region TCR gene segment primarily rearranges to join the Jγ4 and Cγ4 TCR gene segments. TCR Vγ1-expressing cells represent a major population of TCR γδ cells in the adult mouse thymus, peripheral lymphoid tissues, and different epithelia. They constitute a minor population of TCR γδ-positive T cells during fetal and early postnatal life.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566308 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566308 Rev.1
Citations & References
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Development References (4)

  1. Cui ZH, Joetham A, Aydintug MK, Hahn YS, Born WK, Gelfand EW. Reversal of allergic airway hyperreactivity after long-term allergen challenge depends on gammadelta T cells.. Am J Respir Crit Care Med. 2003; 168(11):1324-32. (Clone-specific: Cell separation, Depletion). View Reference
  2. Matsubara S, Takeda K, Jin N, et al. Vgamma1+ T cells and tumor necrosis factor-alpha in ozone-induced airway hyperresponsiveness.. Am J Respir Cell Mol Biol. 2009; 40(4):454-63. (Clone-specific: Flow cytometry). View Reference
  3. Pereira P, Gerber D, Huang SY, Tonegawa S. Ontogenic development and tissue distribution of V gamma 1-expressing gamma/delta T lymphocytes in normal mice.. J Exp Med. 1995; 182(6):1921-30. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Radioimmunoassay). View Reference
  4. Pereira P, Lafaille JJ, Gerber D, Tonegawa S. The T cell receptor repertoire of intestinal intraepithelial gammadelta T lymphocytes is influenced by genes linked to the major histocompatibility complex and to the T cell receptor loci. Proc Natl Acad Sci U S A. 1997; 94(11):5761-5766. (Clone-specific: Flow cytometry). View Reference
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566308 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.