PE Mouse Anti-Human CD56

BD™ PE Mouse Anti-Human CD56

Name: PE Mouse Anti-Human CD56
Brand: BD™
SKU: 347747

Clone MY31

(RUO)

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Product Details

Brand: BD™

Alternative Name: N-CAM; NCAM1; NCAM-1; Neural cell adhesion molecule 1; NKH1; MSK39

Reactivity: Human

Isotype: Mouse BALB/c X C57BL/6 IgG1, κ

Immunogen: KG1a Cell Line

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V NK19

Entrez Gene ID: 4684,916

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody is supplied as 100 µg purified immunoglobulin in 2.0 mL (50 µg/mL) of phosphate-buffered saline. The PE conjugate is supplied as 100 µg in 2.0 mL (50 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent in stable for the period shown on the bottle labeled when stored as directed.

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Antibody Details

MY31

CD56, clone MY31, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from (B6 x BALB/c) F1 mice immunized with the KG1a cell line.

CD56 recognizes an antigen present on natural killer (NK) lymphocytes that is the 140-kdalton (kDa) isoform of the neural cell adhesion molecule (NCAM). The 140-kDa core protein is extensively glycosylated to give a mature antigen.

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Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

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Citations & References

Development References (7)

Edelman G. Cell adhesion molecules. Science. 1983; 219:450-457. (Biology).
Hercend T, Griffin JD, Bensussan A, et al. Generation of monoclonal antibodies to a human natural killer clone. Characterization of two natural killer-associated antigens, NKH1A and NKH2, expressed on subsets of large granular lymphocytes.. J Clin Invest. 1985; 75(3):932-43. (Biology). View Reference
Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991; 146(12):4421-4426. (Biology). View Reference
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). View Reference
Lanier LL, Testi R, Bindl J, Phillips JH. Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule. J Exp Med. 1989; 169(6):2233-2238. (Biology). View Reference
Phillips JH, Lanier LL. Dissection of the lymphokine-activated killer phenomenon: relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis. J Exp Med. 1986; 164(3):814-825. (Biology). View Reference
Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:699-702.

View All (7)

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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.