PE-Cy™7 Mouse Anti-Human CD2

BD™ PE-Cy™7 Mouse Anti-Human CD2

Name: PE-Cy™7 Mouse Anti-Human CD2
Brand: BD™
SKU: 335786

Clone L303.1 (also known as L303)

(RUO)

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Product Details

Brand: BD™

Alternative Name: LFA-2; Lymphocyte function antigen-2; T11/Leu-5; Rosette receptor

Reactivity: Human

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: CD3-activated Human T Lymphocytes

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 5 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

L303.1

• CD2, clone S5.2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T lymphocytes activated by mixed lymphocyte culture.

• CD2, clone L303.1, is derived by the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with CD3-activated human T lymphocytes.

CD2 recognizes a human lymphocyte antigen, 45 to 50 kilodaltons (kDa), which also forms the binding site for sheep erythrocytes.

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Format Details

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-Cy7

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

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Citations & References

Development References (9)

Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin.. Transplantation. 1981; 31(4):283-9. (Biology). View Reference
Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T-cell activation via the physiological ligands for CD2 and the T-cell receptor. J Exp Med. 1988; 168:1145-1156. (Biology).
Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells.. J Immunol. 1999; 163(11):5920-8. (Biology). View Reference
Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.. J Immunol. 1981; 126(6):2117-22. (Biology). View Reference
Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen.. Proc Natl Acad Sci USA. 1990; 87(7):2603-7. (Biology). View Reference
MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
Moingeon P, Chang H, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T-lymphocyte antigen-recognition function. Nature. 1989; 339:312-314. (Biology).

View All (9)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.