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Antibody Cocktail (IL-2 FITC, CD69 PE, CD4 PerCP-Cy™5.5)

BD FastImmune™ Antibody Cocktail (IL-2 FITC, CD69 PE, CD4 PerCP-Cy™5.5)

(RUO)
Product Details
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BD FastImmune™
Human
Flow cytometry
RUO
Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.


Description

Anti-Hu–IL-2, clone 5344.111, is derived from a fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-2.

CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

Anti-Human Interleukin-2 (Anti-Hu–IL-2) recognizes a 15- to 18-kilodalton (kd) glycoprotein.

CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kd and 32-kd chains that are held together by disulfide bridges.

The CD4, antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes.

Preparation And Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340963 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
IL-2 FITC 5344.111 IgG1, κ N/A
CD69 PE 2D7/Basophils IgG1, κ N/A
CD4 PerCP-CY5.5 SK3 IgG1, κ N/A
340963 Rev. 1
Citations & References
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Development References (42)

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340963 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.