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Anti-Human HLA-DR FITC/CD34 PE

Anti-Human HLA-DR FITC/CD34 PE

(RUO)
Product Details
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BD Oncomark™
Human
Flow cytometry
RUO
Phosphate buffered saline with gelatin and 0.1% sodium azide.


Description

Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

CD34 (Anti–HPCA-2), clone 8G12, is derived from hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.

Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kilodaltons (kd), respectively.

The CD34 antigen is a single-chain transmembrane glycoprotein, Mr 105 to 120 kd. The antigen is associated with human hematopoietic progenitor cells and is a differentiation stage–specific leucocyte antigen. Anti–HPCA-1 and Anti–HPCA-2 (clone 8G12) recognize two distinct CD34 epitopes; at least three epitopes have been identified. Clone 8G12 recognizes the class 3 epitope.

Preparation And Storage

Store vials at 2–8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341146 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD34 PE 8G12 IgG1, κ N/A
Anti-HLA DR FITC L243 IgG2a, κ N/A
341146 Rev. 1
Citations & References
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Development References (34)

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  20. Paietta E, Andersen J, Gallagher R, et al. The immunophenotype of acute promyelocytic leukemia (APL): an ECOG study. Leukemia. 1994; 8:1108-1112. (Biology).
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View All (34) View Less
341146 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.