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Anti-Human CD64 PE/CD45 PerCP

Anti-Human CD64 PE/CD45 PerCP

(RUO)
Product Details
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BD Quantibrite™
Human
Fluorescence quantitation
RUO
Buffered saline with BSA and 0.1% sodium azide.


Description

CD64, clone MD22, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c nu/nu mice immunized with FcγRI.

CD45, clone 2D1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

CD64 recognizes the 72-kilodalton (kd) human FcγRI glycoprotein that can bind monomeric IgG.

CD45 (Anti-HLe-1) recognizes human leucocyte antigens, Mr 180 to 220 kd, that are members of the T200 family.

Preparation And Storage

The CD64/CD45 reagent is supplied as a combination of CD64 PE (≥95% 1:1 PE:mAb ratio) and CD45 PerCP in 1 mL of buffered saline with bovine serum albumin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. QuantiBRITE reagents should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340768 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD45 PerCP 2D1 IgG1, κ N/A
CD64 PE MΦP9 IgG2b, κ N/A
340768 Rev. 1
Citations & References
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Development References (25)

  1. Anderson CL, Shen L, Eicher DM, Wewers MD, Gill JK. Phagocytosis mediated by three distinct Fcγ receptor classes on human leukocytes. J Exp Med. 1990; 171:1333-1345. (Biology).
  2. Buckle A-M, Hogg N. The effect of IFN-γ and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J Immunol. 1989; 143:2295-2301. (Biology).
  3. Davis BH, Bigelow NC, Curnutte JT, Ornvold K. Neutrophil CD64 expression: potential diagnostic indicator of acute inflammation and therapeutic monitor of interferon-γ therapy. Lab Hematol. 1995; 1:3-12. (Biology).
  4. Davis BH. Quantitative neutrophil CD64 expression: promising diagnostic indicator of infection or systemic acute inflammatory response. Clin Immunol. 1996; 16:124-129. (Biology).
  5. Debets JMH, van de Winkel JGJ, Ceuppens JL, Dieteren IEM, Buurman WA. Cross-linking of both FcγRI and FcγRII induces secretion of tumor necrosis factor by human monocytes, requiring high affinity Fc-FcγR interactions. J Immunol. 1990; 144:1304-1310. (Biology).
  6. Ernst LK, van de Winkel JGJ, Chiu IM, Anderson CL. Three genes for the human high affinity Fc receptor for IgG (Fc γ RI) encode four distinct transcription products. J Biol Chem. 1992; 267:15692-15700. (Biology).
  7. Fanger MW, Shen L, Graziano RF, Guyre PM. Cytotoxicity mediated by human Fc receptors for IgG. Immunol Today. 1989; 10:92-99. (Biology).
  8. Fanger NA, Wardwell K, Shen L, Tedder TF, Guyre PM. Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells.. J Immunol. 1996; 157(2):541-8. (Biology). View Reference
  9. Gosselin EJ, Wardwell K, Gosselin DR, Alter N, Fisher JL, Guyre PM. Enhanced antigen presentation using human Fc γ receptor (monocyte/macrophage)–specific immunogens. J Immunol. 1992; 149:3477-3481. (Biology).
  10. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
  11. Kerst JM, van de Winkel JGJ, Evans AH, et al. Granulocyte colony-stimulating factor induces hFc γ RI (CD64 antigen)–positive neutrophils via an effect on myeloid precursor cells. Blood. 1993; 6:1457-1464. (Biology).
  12. Krutmann J, Kirnbauer R, Köck A, et al. Cross-linking Fc receptors on monocytes triggers IL-6 production: role in Anti-CD3–induced T cell activation. J Immunol. 1990; 145:1337-1342. (Biology).
  13. Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F. Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells. Blood. 1996; 88:1594-1607. (Biology). View Reference
  14. Perussia B, Dayton ET, Lazarus R, Fanning V, Trinchieri G. Immune interferon induces the receptor for monomeric IgG1 on human monocytic and myeloid cells. J Exp Med. 1983; 158:1092-1113. (Biology).
  15. Petroni KC, Shen L, Guyre PM. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-γ and glucocorticoids. J Immunol. 1988; 140:3467-3472. (Biology).
  16. Repp R, Valerius T, Sendler A, et al. Neutrophils express the high affinity receptor for IgG (FcγRI, CD64) after in vivo application of recombinant human granulocyte colony-stimulating factor. r Blood. 1991; 78:885-889. (Biology).
  17. Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT. Increased phagocyte FcγRI expression and improved Fcγ-receptor–mediated phagocytosis after in vivo recombinant human interferon-γ treatment of normal human subjects. Blood. 1997; 90:3187-3194. (Biology).
  18. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
  19. Shen L, Guyre PM, Fanger MW Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J Immunol. 1987; 534-548. (Biology).
  20. Valerius T, Repp R, de Wit TP, et al. Involvement of the high-affinity receptor for IgG (Fc γ RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy. Blood. 1993; 82:931-939. (Biology).
  21. van Vugt MJ, Heijnen IAFM, Capel PJ, et al. FcR γ-chain is essential for both surface expression and function of human Fc γ RI (CD64) in vivo. Blood. 1996; 87:3593-3599. (Biology).
  22. van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88(6):2358-2361. (Biology). View Reference
  23. van de Winkel JG, Anderson CL. Biology of human immunoglobulin G Fc receptors.. J Leukoc Biol. 1991; 49(5):511-24. (Biology). View Reference
  24. van de Winkel JG, Capel PJ. Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.. Immunol Today. 1993; 14(5):215-21. (Biology). View Reference
  25. van de Winkel JG, Ernst LK, Anderson CL, Chiu IM. Gene organization of the human high affinity receptor for IgG, Fc γ RI (CD64). Characterization and evidence for a second gene. J Biol Chem. 1991; 266:13449-13455. (Biology).
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340768 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.