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Anti-Human CD4 FITC/CD25 PE/CD3 PerCP-Cy™5.5

BD Oncomark™ Anti-Human CD4 FITC/CD25 PE/CD3 PerCP-Cy™5.5

Product Details
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BD Oncomark™
Flow cytometry
Buffered saline with BSA and 0.1% sodium azide.


CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

CD25, clone 2A3, is derived from hybridization of mouse myeloma cell line NS-1 myeloma cells with spleen cells from BALB/c mice immunized with PHA-activated human T lymphocytes.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

The CD4 antigen, Mr 55 kilodalton (kd), is present on T-helper/inducer lymphocytes and monocytes.

CD25 recognizes an antigen that is the human low-affinity interleukin-2 receptor (IL-2R). The antigen has a molecular weight of 55 kd.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

Preparation And Storage

Store vials at 2° to 8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341134 Rev. 1
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Description Clone Isotype EntrezGene ID
CD4 FITC SK3 IgG1, κ 920
CD3 PerCP-Cy5.5 SK7 IgG1, κ N/A
CD25 (IL-2 Receptor α) 2A3 IgG1, κ N/A
341134 Rev. 1
Citations & References
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Development References (25)

  1. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories: T2 protocol. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies : specification, classification, nomenclature = Typage leucocytaire : antigènes de différenciation leucocytaire humains révélés par les anticorps monoclonaux : "Rapports des études communes". Berlin New York: Springer-Verlag; 1984:25-60.
  2. Buckle GJ, Hafler DA, Hollsberg P. HTLV-I-induced T-cell activation. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1996; 13:S107-S113. (Biology).
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  4. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
  5. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  6. Ferrara F, Cimino R, Antinolfi I, et al. Clinical relevance of immunological dissection in T-ALL: a report on 20 cases with stem cell (CD7+, CD4-, CD8-, CD1-) phenotype. Am J Hematol. 1992; 40:98-102. (Biology).
  7. Freedman AS, Nadler LM. Immunologic markers in non-Hodgkin's lymphoma. Hematol Oncol Clin North Am. 1991; 5(5):871-889. (Biology).
  8. Ginaldi L, Matutes E, Farahat N, De Martinis M, Morilla R, Catovsky D. Differential expression of CD3 and CD7 in T-cell malignancies: a quantitative study by flow cytometry. Br J Haematol. 1996; 93:921-927. (Biology).
  9. Hastrup N, Ralfkiaer E, Pallesen G. Aberrant phenotypes in peripheral T cell lymphomas. J Clin Pathol. 1989; 42:398-402. (Biology).
  10. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  11. Hemler ME, Brenner MB, McLean JM, Strominger JL. Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells. Proc Natl Acad Sci USA. 1984; 81:2172-2175. (Biology).
  12. Honda M, Yamamoto N, Nagao S, Tokunaga T. High Levels of soluble interleukin 2 receptors released from human retrovirus-infected cells. Jpn J Cancer Res (Gann). 1987; 78:889-893. (Biology).
  13. Jackson AL, Matsumoto H, Janszen M, Maino V, Blidy A, Shye S. Restricted expression of p55 interleukin 2 receptor (CD25) on normal T cells. Clin Immunol Immunopathol. 1990; 54(1):126-133. (Biology). View Reference
  14. Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
  15. Knowles DM. Immunophenotypic and antigen receptor gene rearrangement analysis in T cell neoplasia. Am J Pathol. 1989; 134:761-785. (Biology).
  16. Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
  17. Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
  18. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  19. Nagatani T, Miyazawa M, Matsuzaki T, et al. Comparative study of cutaneous T-cell lymphoma and adult T-cell leukemia/lymphoma. Semin Dermatol. 1994; 13:216-222. (Biology).
  20. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  21. Robb RJ, Greene WC, Rusk CM. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984; 160(4):1126-1146. (Biology). View Reference
  22. Schwarting R, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:399-402.
  23. Shahabuddin S. Expression and release of IL-2 receptor and production of IL-2 by activated T lymphocyte subsets. J Clin Lab Immunol. 1991; 36:27-32. (Biology).
  24. Teshigawara K, Wang HM, Kato K, Smith KA. Interleukin 2 high-affinity receptor expression requires two distinct binding proteins. J Exp Med. 1987; 165(1):223-238. (Biology). View Reference
  25. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
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341134 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.