PerCP-Cy™5.5 Mouse Anti-Human CD22

BD™ PerCP-Cy™5.5 Mouse Anti-Human CD22

Name: PerCP-Cy™5.5 Mouse Anti-Human CD22
Brand: BD™
SKU: 658329

Clone S-HCL-1 (also known as S-HCL1)

(RUO)

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Product Details

Brand: BD™

Alternative Name: SIGLEC2; SIGLEC-2; Bgp135; BL-CAM; Lyb8; LPAP

Reactivity: Human

Isotype: Mouse IgG2b, κ

Immunogen: Hairy Cell Leukemia Cells and Membranes

Application: Flow cytometry

Concentration: 3 µg/mL

Vol. Per Test: 20 μL

Workshop Number: II B49

Entrez Gene ID: 933

Regulatory Status: RUO

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Antibody Details

S-HCL-1

The CD22 antibody, clone S-HCL-1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from CD-1 mice immunized with whole hairy cell leukemia cells and membrane preparations derived from them.

The CD22 antibody recognizes a 135-kilodalton (kDa) type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD22 antigen is also known as BL-CAM, Bgp135, and Siglec2.

658329 Rev. 1

Format Details

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

658329 Rev.1

Citations & References

Development References (13)

Borowitz MJ, Bousvaros A, Brynes RK, et al. Monoclonal antibody phenotyping of B-cell non-Hodgkin's lymphomas. The Southeastern Cancer Study Group experience.. Am J Pathol. 1985; 121(3):514-21. (Biology). View Reference
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Doody GM, Dempsey PW, Fearon DT. Activation of B lymphocytes: integrating signals from CD19, CD22 and Fc γ RIIb1. Curr Opin Immunol. 1996; 8:378-382. (Biology).
Foon KA, Gale RP, Todd RF. Recent advances in the immunologic classification of leukemia.. Semin Hematol. 1986; 23(4):257-83. (Biology). View Reference
Law CL, Sidorenko SP, Clark EA. Regulation of lymphocyte activation by the cell-surface molecule CD22. Immunol Today. 1994; 15(9):442-449. (Biology). View Reference
Loken MR, Shah VO, Hollander Z, Civin CI. Flow cytometric analysis of normal B lymphoid development.. Pathol Immunopathol Res. 1988; 7(5):357-70. (Biology). View Reference
Mason DY, Stein H, Gerdes J, et al. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid. Blood. 1987; 836-840. (Biology).
Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). View Reference
Terstappen LW, Johnsen S, Segers-Nolten IM, Loken MR. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry.. Blood. 1990; 76(9):1739-47. (Biology). View Reference
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference

View All (13)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.