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CD64 PE
Product Details
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BD™
FCGR1; FcRI; Fc-gamma RI; IgG Fc Receptor I; High affinity IgG FcR1
Human
Mouse BALB/c IgG1, κ
Human Rheumatoid synovial fluid cells and fibronectin-purified monocytes
Flow cytometry
25 μg/mL
20 μL
VI MA36
2209
ASR


644386 Rev. 1
Antibody Details
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10.1

The CD64 antibody, clone 10.1, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from a BALB/c mouse that was immunized first with 2 x 107 rheumatoid synovial fluid cells and on subsequent occasions with 1.5 x 107 fibronectin-purified human monocytes obtained from pools of blood group–matched donors.

CD64 recognizes the 72-kilodalton (kDa) human FcγRI that can bind monomeric IgG.

644386 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
644386 Rev.1
Citations & References
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Development References (8)

  1. Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N. The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur J Immunol. 1987; 17(10):1453-1459. (Biology). View Reference
  2. Ernst LK, van de Winkel JGJ, Chiu IM, Anderson CL. Three genes for the human high affinity Fc receptor for IgG (Fc γ RI) encode four distinct transcription products. J Biol Chem. 1992; 267:15692-15700. (Biology).
  3. Fanger NA, Wardwell K, Shen L, Tedder TF, Guyre PM. Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells.. J Immunol. 1996; 157(2):541-8. (Biology). View Reference
  4. Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F. Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells. Blood. 1996; 88:1594-1607. (Biology). View Reference
  5. van Vugt MJ, Heijnen IAFM, Capel PJ, et al. FcR γ-chain is essential for both surface expression and function of human Fc γ RI (CD64) in vivo. Blood. 1996; 87:3593-3599. (Biology).
  6. van de Winkel JG, Anderson CL. Biology of human immunoglobulin G Fc receptors.. J Leukoc Biol. 1991; 49(5):511-24. (Biology). View Reference
  7. van de Winkel JG, Capel PJ. Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.. Immunol Today. 1993; 14(5):215-21. (Biology). View Reference
  8. van de Winkel JG, Ernst LK, Anderson CL, Chiu IM. Gene organization of the human high affinity receptor for IgG, Fc γ RI (CD64). Characterization and evidence for a second gene. J Biol Chem. 1991; 266:13449-13455. (Biology).
View All (8) View Less
644386 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.