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SPN; Sialophorin; Galactoglycoprotein/GALGP; GPL115; LEUK; Leukosialin; LSN
Mouse IgG1, κ
Flow cytometry
50 μg/mL
20 μL
VI N-L166

655158 Rev. 1
Antibody Details
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The CD43 antibody, clone 1G10, is derived from the hybridization of X63 mouse myeloma cells with spleen cells isolated from mice immunized with a lymph node suspension from a patient with Hodgkin lymphoma.

The CD43 antibody reacts specifically with the major 95–135-kilodalton (kDa) sialoglycoprotein found on most human leucocytes. The CD43 antigen is also known as leukosialin or sialophorin.

655158 Rev. 1
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
655158 Rev.1
Citations & References
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View product citations for antibody "655158" on CiteAb

Development References (12)

  1. Ardman B, Sikorski MA, Staunton DE. CD43 interferes with T-lymphocyte adhesion. Proc Natl Acad Sci U S A. 1992; 89:5001-5005. (Biology).
  2. Bazil V, Strominger JL. CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes. Proc Natl Acad Sci U S A. 1993 May; 90(9):3792-3796. (Biology). View Reference
  3. Bettaieb A, Farace F, Mitjavila MT, et al. Use of a monoclonal antibody (GA3) to demonstrate lineage restricted O-glycosylation on leukosialin during terminal erythroid differentiation. Blood. 1988; 71:1226-1233. (Biology).
  4. Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987; 17(10):1523-1526. (Biology). View Reference
  5. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Horejsi V, Stockinger H. CD43 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:494-497.
  8. Howell DN, Ahuja V, Jones L, Blow O, Sanfilippo FP. Differential expression of CD43 (leukosialin, sialophorin) by mononuclear phagocyte populations. J Leukoc Biol. 1994; 55:536-544. (Biology).
  9. Matsumoto M, Shigeta A, Miyasaka M, Hirata T. CD43 plays both antiadhesive and proadhesive roles in neutrophil rolling in a context-dependent manner. J Immunol. 2008; 181:3628-3635. (Biology).
  10. Nathan C, Xie QW, Halbwachs-Mecarelli L, Jin WW. Albumin inhibits neutrophil spreading and hydrogen peroxide release by blocking the shedding of CD43 (sialophorin, leukosialin). J Cell Biol. 1993; 90:3792-3796. (Biology).
  11. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
  12. van den Berg TK, Nath D, Ziltener HJ, et al. Cutting edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1). J Immunol. 2001; 166:3637-3640. (Biology).
View All (12) View Less
655158 Rev. 1

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Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.


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