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Anti-Human CD8 FITC/CD28 PE

Anti-Human CD8 FITC/CD28 PE

(RUO)
Product Details
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BD Simultest™
Human
Flow cytometry
RUO
Phosphate buffered saline with gelatin and 0.1% sodium azide.


Description

CD8, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

CD28, clone L293, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line.

The CD8 (Leu-2a) antigen is present on the human suppressor/cytotoxic T-lymphocyte subset as well as on a subset of natural killer (NK) lymphocytes. The CD8 antigenic determinant interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. CD8 recognizes an antigen expressed on the 32-kdalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α-subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck.

The CD28 antigen, a disulfide-linked homodimeric glycoprotein (Mr 44 kDa), is a cell adhesion molecule (CAM) and functions as the ligand for the B7/BB-1 antigen that is present on activated B lymphocytes. Interaction of the CD28 antigen with the B7/BB-1 antigen costimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated cytotoxicity.


CD8, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

CD28, clone L293, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line.

The CD8 (Leu-2a) antigen is present on the human suppressor/cytotoxic T-lymphocyte subset as well as on a subset of natural killer (NK) lymphocytes. The CD8 antigenic determinant interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. CD8 recognizes an antigen expressed on the 32-kdalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α-subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck.

The CD28 antigen, a disulfide-linked homodimeric glycoprotein (Mr 44 kDa), is a cell adhesion molecule (CAM) and functions as the ligand for the B7/BB-1 antigen that is present on activated B lymphocytes. Interaction of the CD28 antigen with the B7/BB-1 antigen costimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated cytotoxicity.

Preparation And Storage

The Simultest reagent is supplied as a combination of CD8 FITC and CD28 PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.

340031 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD8 FITC SK1 IgG1, κ N/A
CD28 PE L293 IgG1, κ N/A
340031 Rev. 1
Citations & References
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Development References (23)

  1. Anderson P, Blue ML, Morimoto C, Schlossman SF. Cross-linking of T3 (CD3) with T4 (CD4) enhances the proliferation of resting T lymphocytes. J Immunol. 1987; 139:678-682. (Biology).
  2. Azuma M, Cayabyab M, Buck D, Phillips JH, Lanier LL. CD28 interaction with B7 costimulates primary allogeneic proliferative responses and cytotoxicity mediated by small, resting T lymphocytes.. J Exp Med. 1992; 175(2):353-60. (Biology). View Reference
  3. Damle NK, Mohagheghpour N, Hansen JA, Engleman EG. Alloantigen-specific cytotoxic and suppressor T lymphocytes are derived from phenotypically distinct precursors. J Immunol. 1983; 131:2296-2300. (Biology).
  4. Eichmann K, Johnson J, Falk I, Emmrich F. Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentrations of the T-cell antigen receptor with either Lyt-2 or L3T4. Eur J Immunol. 1987; 17:643-650. (Biology).
  5. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  6. Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
  7. Hansen JA, Martin PJ, Nowinski RC. Monoclonal antibodies identifying a novel T-cell antigen and Ia antigens of human lymphocytes. Immunogenetics. 1980; 10:247-260. (Biology).
  8. Hara R, Fu SM, Hansen JA. Human T-cell activation, II: A new activation pathway used by a major T-cell population via a disulfide-bonded dimer of a 44-kilodalton polypeptide (9-3 antigen). J Exp Med. 1985; 161:1513-1524. (Biology).
  9. June CH, Ledbetter JA, Linsley PS, Thompson CB. Role of the CD28 receptor in T-cell activation. Immunol Today. 1990; 11:211-216. (Biology).
  10. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
  11. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  12. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
  13. Lum LG, Orcutt-Thordarson N, Seigneuret MC, Hansen JA. In vitro regulation of immunoglobulin synthesis by T-cell subpopulations defined by a new human T-cell antigen (9-3). Cell Immunol. 1982; 72:122-129. (Biology).
  14. McMichael AJ, Gotch FM. T-cell antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:31-62.
  15. Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
  16. Morishita Y, Sao H, Hansen JA, Martin PJ. A distinct subset of human CD4+ cells with a limited alloreactive T-cell receptor repertoire. J Immunol. 1989; 143:2783-2789. (Biology).
  17. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  18. Reiter C. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:352-353.
  19. Rotteveel FTM, Kokkelink I, Van Lier RAW, et al. Clonal analysis of functionally distinct human CD4+ T-cell subsets. J Exp Med. 1988; 168:1659-1673. (Biology).
  20. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
  21. Turka LA, Linsley PS, Paine R, Schieven GL, Thompson CB, Ledbetter JA. Signal transduction via CD4, CD8, and CD28 in mature and immature thymocytes: implications for thymic selection. J Immunol. 1991; 146:1428-1436. (Biology).
  22. Van Lier RAW, Brouwer M, DeJong R, Groot M, De Groot E, Aarden L. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:353-355.
  23. van Lier RAW, Brouwer M, Aarden LA. Signals involved in T cell activation, T cell proliferation induced through the synergistic action of anti-CD28 and anti-CD2 monoclonal antibodies. Eur J Immunol. 1988; 18:167-172. (Biology).
View All (23) View Less
340031 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.