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Product Details
BD Pharmingen™
Rat (QC Testing)
Mouse SJL IgG2b, κ
Rat Pooled IgG
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
AB_395121
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures
Purified Mouse Anti-Rat IgG2a (Cat. No. 553893) is routinely tested by ELISA for its ability to recognize and bind Rat IgG2a, kappa (clone R35-95) and Rat IgG2a, lambda (Clone B39-4) while not binding all other Rat Ig isotypes. Biotin Rat Anti-Mouse IgG2b (clone R12-3; Cat. No. 553393) along with a streptavidin reporting system is used for detection.
For sandwich Rat IgG2a ELISA, Biotin Mouse Anti-Rat IgG2a (Cat. No. 553894) is optimal for detection with Purified Mouse Anti-Rat IgG2a (B46-7; Cat. No. 553918) for capture. RG7/1.30 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2a, we recommend FITC Mouse Anti-Rat IgG2a (Cat. No. 553896).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
553893 Rev. 13
Antibody Details
RG7/1.30
The Rg7/1.30 antibody reacts specifically with the Fc region of rat IgG2a. It does not react with other Ig isotypes.
553893 Rev. 13
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553893 Rev.13
Citations & References
View product citations for antibody "553893" on CiteAb
Development References (1)
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Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen). View Reference
553893 Rev. 13
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.