Flow cytometric analysis for CD83 in Rhesus macaque cultured dendritic cells. Peripheral blood mononuclear cells (PBMC) from Rhesus macaque were treated with 20 ng/mL recombinant human IL-4 (Cat. No. 554605), 20 ng/mL recombinant human TNF (Cat. No. 554618) and 20 ng/mL recombinant human GM-CSF (Cat. No. 550068) for 7 days at 37°C. Cells were then stained with either a FITC Mouse IgG1, κ isotype control (shaded) or with the FITC Mouse Anti-Human CD83 antibody (unshaded). Histograms were derived from gated events based on light scattering characteristics for dendritic cells. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
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Flow cytometric analysis for CD83 in Rhesus macaque cultured dendritic cells. Peripheral blood mononuclear cells (PBMC) from Rhesus macaque were treated with 20 ng/mL recombinant human IL-4 (Cat. No. 554605), 20 ng/mL recombinant human TNF (Cat. No. 554618) and 20 ng/mL recombinant human GM-CSF (Cat. No. 550068) for 7 days at 37°C. Cells were then stained with either a FITC Mouse IgG1, κ isotype control (shaded) or with the FITC Mouse Anti-Human CD83 antibody (unshaded). Histograms were derived from gated events based on light scattering characteristics for dendritic cells. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Product Details
BD Pharmingen™
BL11; HB15; B-cell activation protein
Rhesus, Cynomolgus, Baboon (QC Testing)
Mouse IgG1, κ
Human CD83 transfected COS cells
Flow cytometry (Routinely Tested)
20 µl
VI
9308
AB_1727516
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
FITC Mouse IgG1, κ Isotype Control RUO
Size
50 Tests
Cat No.
556649
Recombinant Human IL-4 RUO
Size
5 µg
Cat No.
554605
Recombinant Human TNF RUO
Size
10 µg
Cat No.
554618
Recombinant Human GM-CSF RUO
Size
10 µg
Cat No.
550068
560553 Rev. 2
Antibody Details
HB15e
The HB15e monoclonal antibody specifically binds to a 45 kDa type 1 transmembrane glycoprotein member of the Ig superfamily. CD83 is composed of a single V-type Ig extracellular domain with a C-terminal cytoplasmic tail. Cell surface CD83 is expressed mainly by follicular dendritic cells, circulating dendritic cells, interdigitating dendritic cells in lymphoid tissues, in vitro-generated dendritic cells and thymic dendritic cells. However, its expression is not restricted to dendritic cells. CD83 is also expressed on some germinal center B cells and some lymphoblastoid cell lines. Although its function is not known, it may play a role in cell-cell interaction during antigen presentation.
560553 Rev. 2
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
560553 Rev.2
Citations & References
Development References (8)
-
Hart DN. Dendritic cells: unique leukocyte populations which control the primary immune response. Blood. 1997; 90(9):3245-3287. (Biology). View Reference
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
-
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Summers KL, Daniel PB, O'Donnell JL, Hart DN. Dendritic cells in synovial fluid of chronic inflammatory arthritis lack CD80 surface expression. Clin Exp Immunol. 1995; 100(1):81-89. (Biology). View Reference
-
Weissman D, Li Y, Ananworanich J, et al. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1. Proc Natl Acad Sci U S A. 1995; 92(3):826-830. (Biology). View Reference
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Zhou LJ, Schwarting R, Smith HM, Tedder TF. A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily. J Immunol. 1992; 149(2):735-742. (Biology). View Reference
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Zhou LJ, Tedder TF. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J Immunol. 1995; 154(8):3821-3835. (Biology). View Reference
560553 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
