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- BD® AbSeq Assay
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
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- Nature Research Academies Workshop 2023
- CYTO 2023: Advancing the World of Cytometry
- Singapore Gene & Cell Therapy Conference 2023
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- Nature Research Masterclass 2023
- Novel Approaches to Single-Cell Plant Research: from Real-Time Imaging Cell Sorting to Single-Nuclei Transcriptomics
- Advances in Immune Monitoring Series
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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
ELISA: For detection of rat IgG1, G2a, G2b, G2c, IgA, or IgM in a sandwich ELISA, biotinylated mAb MRK-1 (can be cocktailed with biotin-conjugated anti-rat Ig λ light chain, clone G33-11) is optimal for detection with purified anti-rat Ig isotype-specific mAbs (RG11/39.4, Cat. No. 553889; RG7/1.30, Cat. No. 553893; RG7/11.1, Cat. No. 553897 or G15-337, Cat. No. 553882; A92-1, [available by custom order]; A93-2, [available by custom order]; G53-238, Cat. No. 553885) for capture, respectively.
Flow Cytometry: Biotin-conjugated MRK-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining.
Immunohistochemistry: For use as a second-step in IHC, we suggest RG7/7.6 mAb (anti-rat and -hamster Ig κ light chain) in our special formulation for immunohistochemistry, Cat. No. 550336.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
The MRK-1 monoclonal antibody reacts specifically with rat Igs bearing κ light chains. It does not react with rat immunoglobulin λ light chains or heavy chains. The MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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