Alexa Fluor® 647 Rat anti-Mouse CD160

BD Pharmingen™ Alexa Fluor® 647 Rat anti-Mouse CD160

Name: Alexa Fluor® 647 Rat anti-Mouse CD160
Brand: BD Pharmingen™
SKU: 562217

Clone CNX46-3

(RUO)

Multicolor flow cytometric analysis of CD160 expression on mouse spleen cells. Spleen cells from a C57BL/6 mouse were stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062) and PE Mouse Anti-Mouse NK1.1 (Cat. No. 553165) antibodies and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dotted line histogram) or Alexa Fluor® 647 Rat Anti-Mouse CD160 antibody (Cat. No. 562217; solid line histogram). Flow cytometric fluorescence histograms showing the expression of CD160 (or Ig Isotype Control background staining) by CD3-NK1.1+ NK cells (Left Panel) or CD3+NK1.1+ T cells (Right Panel) were generated for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Cd160; By55; Natural killer cell receptor BY55

Reactivity: Mouse (QC Testing)

Isotype: Rat F344, also known as Fischer, CDF IgG2a, κ

Immunogen: Mouse CD160 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10924600

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 557690

FITC Hamster Anti-Mouse CD3e RUO

Size 0.5 mg Cat No. 553062

PE Mouse Anti-Mouse NK-1.1 RUO

Size 0.2 mg Cat No. 553165

562217 Rev. 1

Antibody Details

CNX46-3

The CNX46-3 monoclonal antibody specifically binds to mouse CD160, also known as Natural Killer Cell Receptor BY55. CD160 is a glycosyolphosphatidylinositol-anchored membrane glycoprotein that contains a single Ig domain. This member of the Ig Superfamily was the first identified as a MHC Class I antigen-specific Ig-like Receptor that was expressed by mouse NK cells. As a receptor, CD160 can bind to classical and non-classical MHC class I molecules with low affinity. As a ligand, CD160 can bind to HVEM (Herpes Virus Entry Mediator, a TNF Receptor Family Member). CD160 is expressed on a subset of NK cells, NKT cells, activated CD8+ T cells and TCR γδ T cells. The functions of CD160 have been reported to regulate NK cell activation both positively and negatively, depending on stimulus.

562217 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

562217 Rev.1

Citations & References

Development References (2)

Anumanthan A, Bensussan A, Boumsell L, et al. Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998; 161(6):2780-2790. (Biology: Flow cytometry, Immunoprecipitation, Western blot). View Reference
Maeda M, Carpenito C, Russell RC, et al. Murine CD160, Ig-like receptor on NK cells and NKT cells, recognizes classical and nonclassical MHC class I and regulates NK cell activation. J Immunol. 2005; 175(7):4426-4432. (Immunogen). View Reference

562217 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.