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BD™ Anti-Human Lineage Cocktail 2 (lin 2) (CD3, CD14, CD19, CD20, CD56)
(RUO (GMP))Anti-Human Lineage Cocktail 2 (lin 2) (CD3, CD14, CD19, CD20, CD56)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
The following antibody clones are each composed of mouse IgG1 heavy chains and kappa light chains: CD3, clone SK7 CD19, clone SJ25C1 CD20, clone L27 The following antibody clones are each composed of mouse IgG2b heavy chains and kappa light chains: CD14, clone MφP9 CD56, clone NCAM16.2
The Lineage Cocktail 2 (lin 2) contains antibody clones that, in combination, stain lymphocytes, monocytes, eosinophils, and neutrophils. It includes CD3, CD14, CD19, CD20, and CD56. Peripheral blood dendritic cells (DCs), including those expressing CD16, can be distinguished from other leucocytes by their lack of staining with lin 2.
Description | Clone | Isotype | EntrezGene ID |
---|---|---|---|
CD3 FITC | SK7 | IgG1, κ | N/A |
CD14 FITC | MΦP9 | IgG2b, κ | N/A |
CD19 FITC | SJ25C1 | IgG1, κ | N/A |
CD20 FITC | L27 | IgG1, κ | N/A |
CD56 FITC | NCAM16.2 | IgG2b, κ | N/A |
Development References (10)
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Banchereau J, Schmitt D, eds. Dendritic Cells in Fundamental and Clinical Immunology, Volume 2. 1995. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Freudenthal PS, Steinman RM. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc Natl Acad Sci USA. 1990; 87:7698-7702. (Biology).
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MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
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Markowicz S, Engleman EG. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest. 1990; 85:955-961. (Biology).
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Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
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Piccioli D, Tavarini S, Borgogni E, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. 2007; 109(12):5371-5379. (Biology).
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Signorino E, Brusa D, Granata R, Malavasi F, Ferrone S, Matera L. Contribution of Dendritic Cells FcγRI and FcγRIII to Cross-Presentation of Tumor Cells Opsonized with the Anti-MHC Class I Monoclonal Antibodies. Cancer Biol Ther. 6(12)(Biology).
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Steinman R M. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 1991; 9:271-296. (Biology).
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
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