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Purified Mouse Anti-Human TRAF2
Purified Mouse Anti-Human TRAF2
Western blot analysis of TRAF2. Lysates from Jurkat human T cells were probed with anti-human TRAF2 (clone C90-481) at 5.0 µg/ml (lane 1), 2.0 µg/ml (lane 2), and 1.0 µg/ml (lane 3). TRAF2 was detected at ~53 kDa
Western blot analysis of TRAF2. Lysates from Jurkat human T cells were probed with anti-human TRAF2 (clone C90-481) at 5.0 µg/ml (lane 1), 2.0 µg/ml (lane 2), and 1.0 µg/ml (lane 3). TRAF2 was detected at ~53 kDa
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2a, κ
Human TRAF2 aa. 93-199
Western blot (Routinely Tested)
53 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2 µg/ml). Human cell lines including Jurkat T cells (ATCC TIB-152), 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and Daudi Burkitt lymphoma cells (ATCC CCL-213) are recommended as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558890 Rev. 4
Antibody Details
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TRAFs (TNF receptor associated factors) are signal transducing molecules which participate in forming TNF/Nerve Growth Factor receptor-associated signalling complexes. TRAF proteins share a novel C-terminal homology  region, the "TRAF" domain, a coiled-coiled region and the majority have Zinc/RING finger motifs. At least six TRAF proteins (TRAFs 1-6) have been identified. These proteins are cytoplasmic adapters; they bind to cytoplasmic domains of various receptors and can function to recruit other proteins to a signaling complex thereby promoting intracellular signal transduction. Specifically, TRAF2 has been shown to interact with the following surface receptors: TNFRII, CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1. TRAF2 has also been shown to associate with intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK. Thus, the interaction of TRAF2 with these various receptors and intracellular proteins plays an important role modulating downstream events which can result in inducing cell death or keeping cells alive. For example, cells deficient in TRAF2 demonstrated increased sensitivity to TNF-induced cell death, but a decrease in TNF-induced JNK activation, and little change on NF-kB activation.  TRAF2 is detected at ~ 53 kDa in western blot analysis. The C90-481 antibody reacts with human TRAF2.  A synthetic protein freagment corresponding to amino acids 93-199 of human TRAF2 was used as immunogen.  

558890 Rev. 4
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
558890 Rev.4
Citations & References
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Development References (6)

  1. Arch RH, Gedrich RW, Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs)--a family of adapter proteins that regulates life and death. Genes Dev. 1998; 12(18):2821-2830. (Biology). View Reference
  2. Hsu H, Shu HB, Pan MG, Goeddel DV. TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways. Cell. 1996; 84(2):299-308. (Biology). View Reference
  3. Rothe M, Sarma V, Dixit VM, Goeddel DV. TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40. Science. 1995; 269(5229):1424-1427. (Biology). View Reference
  4. Shu HB, Takeuchi M, Goeddel DV. The tumor necrosis factor receptor 2 signal transducers TRAF2 and c-IAP1 are components of the tumor necrosis factor receptor 1 signaling complex. Proc Natl Acad Sci U S A. 1996; 93(24):13973-13978. (Clone-specific: Western blot). View Reference
  5. Tsitsikov EN, Wright DA, Geha RS. CD30 induction of human immunodeficiency virus gene transcription is mediated by TRAF2. Proc Natl Acad Sci U S A. 1997; 94(4):1390-1395. (Biology). View Reference
  6. Yeh WC, Shahinian A, Speiser D. Early lethality, functional NF-kappaB activation, and increased sensitivity to TNF-induced cell death in TRAF2-deficient mice. Immunity. 1997; 7(5):715-725. (Clone-specific: Activation). View Reference
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558890 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.