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Purified Mouse Anti-Human TRADD
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Human TRADD aa. 14-62
Western blot (Routinely Tested)
36 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Daudi B lymphoma cells (ATCC CRL-213) and Jurkat T cells (ATCC TIB-152) are suggested as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556496 Rev. 5
Antibody Details
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TRADD (TNF Receptor Associated Death domain) is an ~36 kDa protein that interacts specifically with the cytoplasmic domain of the type I TNF Receptor. Overexpression of the C-terminal 118 amino acids of TRADD, the "death domain," is sufficient to induce two major TNF-induced responses, apoptosis and activation of nuclear transcription factor, NF-κB.  Evidence suggests that TRADD interacts with other signal molecules including TRAF1, TRAF2 and FADD, allowing recruitment of these molecules to the TNF receptor complex. A similar interaction has been demonstrated for TRADD and the serine/threonine kinase RIP.  Coexpression of the ICE-specific protease inhibitor, CrmA, inhibits TRADD-induced apoptosis, but does not affect induction of NF-κB, suggesting that TRADD may serve to initiate distinct signal pathways.

The B36-2 antibody recognizes an ~36 kDa band corresponding to human TRADD.  A recombinant human TRADD protein fragment corresponding to amino acids 14-62 was used as immunogen.

556496 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
556496 Rev.5
Citations & References
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View product citations for antibody "556496" on CiteAb

Development References (3)

  1. Hsu H, Shu HB, Pan MG, Goeddel DV. TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways. Cell. 1996; 84(2):299-308. (Biology). View Reference
  2. Hsu H, Xiong J, Goeddel DV. The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation. Cell. 1995; 81(4):495-504. (Biology). View Reference
  3. Varfolomeev EE, Boldin MP, Goncharov TM, Wallach D. A potential mechanism of "cross-talk" between the p55 tumor necrosis factor receptor and Fas/APO1: proteins binding to the death domains of the two receptors also bind to each other. J Exp Med. 1996; 183(3):1271-1275. (Biology). View Reference
556496 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.