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BD Transduction Laboratories™ Purified Mouse Anti-Ral A
Clone 8/Ral A (RUO)
Western blot analysis of Ral A on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-Ral A antibody.
Immunofluorescence staining of rat neurons at 5 µg/ml of the anti-Ral A antibody.
Western blot analysis of Ral A on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-Ral A antibody.
Immunofluorescence staining of rat neurons at 5 µg/ml of the anti-Ral A antibody.
Product Details
BD Transduction Laboratories™
Rat (QC Testing), Dog, Chicken (Tested in Development)
Mouse IgG2a, κ
Human Ral A aa. 35-206
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunoprecipitation (Not Recommended)
24-28 kDa
250 µg/ml
AB_397618
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
610221 Rev. 3
Antibody Details
8/Ral A
Ral is a low molecular weight GTP-binding protein belonging to the Ras superfamily of GTP-binding proteins and shows 50% amino acid identity to Ras. Ral cDNA clones have been isolated from human placenta, human pheochromocytoma, simian ß-lymphocyte, and marine ray electric lobe cDNA libraries. In humans, cDNA sequences for Ral A and RalB have been determined. The predicted amino acid sequences show 85% identity. Both proteins consist of 206 amino acids with a predicted molecular weight of 24kDa. However, Ral A isolated from human platelets shows an apparent molecular weight of 26-28kDa. Ral A mRNA levels in adult mouse tissues appear to be highest in testes, ovary, and brain, with lower levels found in the liver, spleen, kidney, thymus, heart, and salivary glands. Similar results have been observed in rat tissues.
610221 Rev. 3
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610221 Rev.3
Citations & References
View product citations for antibody "610221" on CiteAb
Development References (5)
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Goi T, Rusanescu G, Urano T, Feig LA. Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth. Mol Cell Biol. 1999; 19(3):1731-1741. (Biology: Immunofluorescence). View Reference
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Kinashi T, Katagiri K, Watanabe S, Vanhaesebroeck B, Downward J, Takatsu K. Distinct mechanisms of alpha 5beta 1 integrin activation by Ha-Ras and R-Ras. J Biol Chem. 2000; 275(29):22590-22596. (Biology: Western blot). View Reference
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Olofsson B, Chardin P, Touchot N, Zahraoui A, Tavitian A. Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues. Oncogene. 1988; 3(2):231-234. (Biology). View Reference
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Polakis PG, Weber RF, Nevins B, Didsbury JR, Evans T, Snyderman R. Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. J Biol Chem. 1989; 264(28):16383-16389. (Biology). View Reference
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Xu L, Frankel P, Jackson D, et al. Elevated phospholipase D activity in H-Ras- but not K-Ras-transformed cells by the synergistic action of RalA and ARF6. Mol Cell Biol. 2003; 23(2):645-654. (Biology: Immunoprecipitation, Western blot). View Reference
610221 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.