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Purified Mouse Anti-Human vWF
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Immunohistochemistry-frozen (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555849 Rev. 9
Antibody Details
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Reacts with von Willebrand Factor (vWF), a multimeric plasma glycoprotein synthesized by endothelial cells and megakaryocytes. In plasma, vWF circulates as multimers which range from dimers of about 500 kDa to multimers of 20000 kDa. It functions as a carrier for Factor VIII and is a mediator of initial platelet adhesion to subendothelium and platelet-platelet interaction. Platelets bind to vWF through the platelet GPIb/IX (CD42b/CD42a) and GPIIb/IIIa (CD41/CD61) complexes This antibody is effective in detecting vWF by ELISA, with no evidence of cross reactivity with fibronectin or fibrinogen.

555849 Rev. 9
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555849 Rev.9
Citations & References
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Development References (2)

  1. Ginsburg D, Bockenstedt PL, Allen EA, et al. Fine mapping of monoclonal antibody epitopes on human von Willebrand factor using a recombinant peptide library. Thromb Haemost. 1991; 67(1):166-171. (Clone-specific: Immunohistochemistry). View Reference
  2. Sadler JE. von Willebrand factor. J Biol Chem. 1991; 266(34):22777-22780. (Clone-specific: Immunohistochemistry). View Reference
555849 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.