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      Purified Mouse Anti-Human CEACAM5 (CD66e)
      Purified Mouse Anti-Human CEACAM5 (CD66e)
      Immunohistochemical analysis of CEACAM5 (CD66e) expression    Left Panel - Immunohistochemical staining of Human Normal Colon. Formalin-fixed, paraffin-embedded sections of Human Normal Colon were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; Left Image) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; Right Image) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337] and Streptavidin HRP (Cat. No.550946). Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Original magnification 20x.    Right Panel - Immunohistochemical staining of Human Colon Cancer. Formalin-fixed, paraffin-embedded sections of Human Colon Cancer were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; Left Image) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; Right Image) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337] and Streptavidin HRP (Cat. No.550946). Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Original magnification 20x.
      Purified Mouse Anti-Human CEACAM5 (CD66e)
      Flow cytometric analysis of CEACAM5 (CD66e) expression on BxPC-3 cells. Cells from the human BxPC-3 (Adenocarcinoma, ATCC CRL-1687™) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dotted line histogram) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; solid line histogram) at 0.5 µg/test, followed by Biotin Goat Anti-Mouse Ig (Cat. No. 550337/553999) at 0.25 µg/test followed by PE Streptavidin (Cat. No. 554061). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CEACAM5 (CD66e) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
      Purified Mouse Anti-Human CEACAM5 (CD66e) Purified Mouse Anti-Human CEACAM5 (CD66e)
      Immunohistochemical analysis of CEACAM5 (CD66e) expression    Left Panel - Immunohistochemical staining of Human Normal Colon. Formalin-fixed, paraffin-embedded sections of Human Normal Colon were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; Left Image) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; Right Image) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337] and Streptavidin HRP (Cat. No.550946). Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Original magnification 20x.    Right Panel - Immunohistochemical staining of Human Colon Cancer. Formalin-fixed, paraffin-embedded sections of Human Colon Cancer were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; Left Image) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; Right Image) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337] and Streptavidin HRP (Cat. No.550946). Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Original magnification 20x.
      Flow cytometric analysis of CEACAM5 (CD66e) expression on BxPC-3 cells. Cells from the human BxPC-3 (Adenocarcinoma, ATCC CRL-1687™) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dotted line histogram) or Purified Mouse Anti-CEACAM5 (CD66e) antibody (Cat. No. 568709; solid line histogram) at 0.5 µg/test, followed by Biotin Goat Anti-Mouse Ig (Cat. No. 550337/553999) at 0.25 µg/test followed by PE Streptavidin (Cat. No. 554061). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CEACAM5 (CD66e) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
      Product Details
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      BD Pharmingen™
      CD66e; CEACAM-5; CEACAM5; Carcinoembryonic antigen-related cell adhesion molecule 5
      Human (QC Testing)
      Mouse IgG1, κ
      CEACAM5 Protein
      Flow cytometry (Routinely Tested), Immunohistochemistry (Tested During Development)
      0.5 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      568709 Rev. 1
      Antibody Details
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      CB30

      The CB30 monoclonal antibody specifically recognizes Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) which is also known as CD66e. CEACAM5 (CD66e) is an ~100-200 kDa cell surface glycoprotein that is comprised of an N-terminal IgV-like domain followed by six IgC-like domains which is glycosylphosphatidylinositol (GPI)-linked to the membrane. This adhesion molecule presents as a homodimer that is encoded by CEACAM5 (CEA cell adhesion molecule 5) which belongs to the carcinoembryonic antigen (CEA) gene family within the Ig gene superfamily. CEACAM5 (CD66e) is expressed by epithelial cells and mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules. It is involved in intracellular signaling and may play a role in tumor cell  progression and metastasis.  CEACAM5 (CD66e) can be overexpressed in many cancers and tumor cell lines.

      568709 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      568709 Rev.1
      Citations & References
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      View product citations for antibody "568709" on CiteAb

      Development References (9)

      1. Blumenthal RD, Hansen HJ, Goldenberg DM. Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen).. Cancer Res. 2005; 65(19):8809-17. (Biology). View Reference
      2. Horenstein AL, Crivellin F, Funaro A, Said M, Malavasi F. Design and scaleup of downstream processing of monoclonal antibodies for cancer therapy: from research to clinical proof of principle.. J Immunol Methods. 2003; 275(1-2):99-112. (Biology). View Reference
      3. Kalinina T, Güngör C, Thieltges S, et al. Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.. BMC Cancer. 2010; 10:295. (Biology). View Reference
      4. Knutson S, Raja E, Bomgarden R, et al. Development and Evaluation of a Fluorescent Antibody-Drug Conjugate for Molecular Imaging and Targeted Therapy of Pancreatic Cancer.. PLoS One. 2016; 11(6):e0157762. (Biology). View Reference
      5. Pakdel A, Naghibalhossaini F, Mokarram P, Jaberipour M, Hosseini A. Regulation of carcinoembryonic antigen release from colorectal cancer cells.. Mol Biol Rep. 2012; 39(4):3695-704. (Biology). View Reference
      6. Pavoni E, Flego M, Dupuis ML, et al. Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein.. BMC Cancer. 2006; 6:41. (Biology). View Reference
      7. Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H. Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.. PLoS One. 2010; 5(1):e8747. (Biology). View Reference
      8. Wakabayashi-Nakao K, Hatakeyama K, Ohshima K, Ken Yamaguchi K, Mochizuki T. Carcinoembryonic antigen-related cell adhesion molecule 4 (CEACAM4) is specifically expressed in medullary thyroid carcinoma cells.. Biomed Res. 2014; 35(4):237-42. (Biology). View Reference
      9. Zheng C, Feng J, Lu D, et al. A novel anti-CEACAM5 monoclonal antibody, CC4, suppresses colorectal tumor growth and enhances NK cells-mediated tumor immunity.. PLoS One. 2011; 6(6):e21146. (Biology). View Reference
      View All (9) View Less
      568709 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.