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Purified Mouse Anti-PP5
Purified Mouse Anti-PP5
Western blot analysis of PP5 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-PP5 antibody.
Purified Mouse Anti-PP5

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Western blot analysis of PP5 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-PP5 antibody.

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Rat PP5 aa. 36-238
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
58 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611020 Rev. 1
Antibody Details
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Phosphorylation and dephosphorylation on serine and threonine residues is critical for signal transduction and the regulation of numerous cellular functions.  Phosphorylation levels are modulated by protein kinases and phosphatases.  The Ser/Thr phosphatase PP5 (also known as PPT) is a model protein of the fourth subfamily of the PPP-family of phosphatases which includes PP1, PP2A, and PP2B.  PP5 is primarily located in the nucleus, demonstrates widespread tissue distribution, and is highly conserved among species.  It consists of a C-terminal catalytic domain and an N-terminal TPR (tetratricopeptide repeat) domain which is involved in protein-protein interactions.  The TPRs of PP5 mediate protein phosphatase activity.  TPRs mediate the interaction of PP5 with various proteins including the hsp90-glucocorticoid receptor complex and the kinase domain of  ANP-guanylate cyclase receptor.  PP5 is thought to be important in cell growth.  For example, it functions upstream of p53 to regulate p21/WAF1/Cip1-mediated G1 growth arrest.  Thus, PP5 is a nuclear protein phosphatase that is involved in signaling pathways of cell growth and arrest.  

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611020 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611020 Rev.1
Citations & References
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Development References (5)

  1. Becker W, Kentrup H, Klumpp S, Schultz JE, Joost HG. Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain. J Biol Chem. 1994; 269(36):22586-225920. (Biology). View Reference
  2. Chen MX, McPartlin AE, Brown L, Chen YH, Barker HM, Cohen PT. A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus. EMBO J. 1994; 13(18):4278-4290. (Biology). View Reference
  3. Das AK, Cohen PW, Barford D. The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions. EMBO J. 1998; 17(5):1192-1199. (Biology). View Reference
  4. Ollendorff V, Donoghue DJ. The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J Biol Chem. 1997; 272(51):32011-32018. (Biology). View Reference
  5. Zuo Z, Dean NM, Honkanen RE. Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21(WAF1/Cip1) and mediate growth arrest. J Biol Chem. 1998; 273(20):12250-12258. (Biology). View Reference
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611020 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.