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Western blot analysis of PI3-Kinase p110δ expression on rat embryonic (E21) cerebrum lysate. Rat cerebrum lysate (Cat. No. 611463) was stained with Purified Mouse Anti-PI3 Kinase p110δ (Cat. No. 611014) at 1:500 (Lane 1), 1:1000 (Lane 2), and 1:2000 (Lane 3) dilutions. PI3-Kinase p110δ expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).
Immunofluorescence staining of human endothelial cells. Human endothelial cells were stained with Purified Mouse Anti-PI3 Kinase p110δ, then visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).
BD Transduction Laboratories™ Purified Mouse Anti-PI3 Kinase p110δ
BD Transduction Laboratories™ Purified Mouse Anti-PI3 Kinase p110δ
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: For additional technical guidance, please refer to the protocols under "Cell Biology (WB, IP, IHC, IF)" at our website:
http://www.bdbiosciences.com/us/s/resources.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Phosphoinositide 3 Kinase (PI3 Kinase) phosphorylates the D-3 position of the inositol ring of phosphatidylinositol, producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns (3,4,5)P3. PI3-kinase is a heterodimer of an 85 kDa regulatory subunit (p85) containing two SH2 domains and an SH3 domain, and a 110 kDa catalytic subunit (p110). Four isoforms of p110 have been identified (α , β , γ, and δ). The p110α, β isoforms are distributed ubiquitously. The p110δ isoform is found exclusively in lymphocytes and lymphoid tissues. Lymphocytes also contains p110α and both isoforms interact with p85 and are recruited to activated signaling complexes. However, several biochemical and structural differences distinguish p110δ. Unlike p110α, p110δ autophosphorylates and does not phosphorylate p85. In addition, p110δ contains a Pro-rich region, which endows it with the potential for interaction with SH3 domain-containing proteins, and a bZIP-like domain, which is a common transcription factor domain that has also been identified in certain protein kinases. With the established role of PI3-Ks in cytoskeletal rearrangements, it is thought that p110δ contributes specifically to the regulation of lymphocytes extravasation into tissues. There is a high degree of homology in the immunogen region with the P110β form (77%), and it is likely that our antibody will detect both the beta and delta forms.
Development References (1)
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Chantry D, Vojtek A, Kashishian A, et al. p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J Biol Chem. 1997; 272(31):19236-19241. (Biology). View Reference
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