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Purified Mouse Anti- NuMA
Purified Mouse Anti- NuMA

Western blot analysis of NuMA on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- NuMA antibody.  NuMA has been reported to have a calculated molecular weight of 238 kDa, but may be observed to be migrating in a range between

238-290 kDa in HeLa whole cell extracts.

Purified Mouse Anti- NuMA

Immunofluorescence staining for NuMA in rabbit kidney.

Western blot analysis of NuMA on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- NuMA antibody.  NuMA has been reported to have a calculated molecular weight of 238 kDa, but may be observed to be migrating in a range between

238-290 kDa in HeLa whole cell extracts.

Immunofluorescence staining for NuMA in rabbit kidney.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog (Tested in Development)
Mouse IgM
Human NuMA aa. 10-189
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
238-290 kDa
250 µg/ml
AB_397914
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610561 Rev. 2
Antibody Details
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22/NuMA

NuMA (Nuclear Mitotic Apparatus protein) is a 2115 amino acid protein with a coiled-coil structure similar to that of myosins and intermediate filaments. Indirect immunofluorescence assays indicate that NuMA's localization is very dynamic. During interphase, NuMA is in the nucleus and during mitosis it moves to the polar regions of the mitotic spindle. NuMA is a very abundant phosphoprotein and antibodies to this protein are often found in patients with autoimmune diseases. Although NuMA is thought to be a structural component of the nucleus, the precise cellular function for this protein is still unknown.

610561 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610561 Rev.2
Citations & References
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Development References (4)

  1. Elbi C, Misteli T, Hager GL. Recruitment of dioxin receptor to active transcription sites. Mol Biol Cell. 2002; 13(6):2001-2015. (Biology: Immunofluorescence). View Reference
  2. Munnia A, Schutz N, Romeike BF, et al. Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor. Oncogene. 2001; 20(35):4853-4863. (Biology: Immunofluorescence). View Reference
  3. Steen RL, Cubizolles F, Le Guellec K, Collas P. A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract. J Cell Biol. 2000; 149(3):531-536. (Biology: Immunofluorescence). View Reference
  4. Yang CH, Lambie EJ, Snyder M. NuMA: an unusually long coiled-coil related protein in the mammalian nucleus. J Cell Biol. 1992; 116(6):1303-1317. (Biology). View Reference
View All (4) View Less
610561 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.