BD Transduction Laboratories™ Purified Mouse Anti-Glutamine Synthetase
Clone 6/Glutamine Synthetase
(RUO)
Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody. Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visibile staining of astrocytes in the section. Magnification: 40X. Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™ 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).
Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody. Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visibile staining of astrocytes in the section. Magnification: 40X. Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™ 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).
Product Details
BD Transduction Laboratories™
Glutamate-Ammonia Ligase; GLUL; GLNS
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2a
Human Glutamine Synthetase aa. 1-373
Western blot (Routinely Tested), Bioimaging, Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_397880
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Triton is a trademark of the Dow Chemical Company.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
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Rat Cerebrum Lysate RUO
Size
500 µg
Cat No.
611463
HRP Goat Anti-Mouse Ig RUO
Size
1 mL
Cat No.
554002
610518 Rev. 3
Antibody Details
6/Glutamine Synthetase
Glutamine synthetase catalyzes the amination of glutamic acid to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
610518 Rev. 3
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610518 Rev.3
Citations & References
Development References (5)
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Fei ZL, D'Ambrosio C, Li S, Surmacz E, Baserga R. Association of insulin receptor substrate 1 with simian virus 40 large T antigen. Mol Cell Biol. 1995; 15(8):4232-4239. (Biology: Immunoprecipitation). View Reference
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Kentroti S, Baker R, Lee K, Bruce C, Vernadakis A. Platelet-activating factor increases glutamine synthetase activity in early and late passage C-6 glioma cells. J Neurosci Res. 1991; 28(4):497-506. (Biology). View Reference
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Kronfeld I, Kazimirsky G, Lorenzo PS, Garfield SH, Blumberg PM, Brodie C. Phosphorylation of protein kinase Cdelta on distinct tyrosine residues regulates specific cellular functions. J Biol Chem. 2000; 275(45):35491-35498. (Biology: Western blot). View Reference
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Labow BI, Souba WW, Abcouwer SF. Glutamine synthetase expression in muscle is regulated by transcriptional and posttranscriptional mechanisms. Am J Physiol. 1999; 276(6):E1136-E1145. (Biology: Western blot). View Reference
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Vardimon L, Fox LE, Cohen-Kupiec R, Degenstein L, Moscona AA. Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase. Mol Cell Biol. 1991; 11(10):5275-5284. (Biology). View Reference
610518 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
