Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Errors in DNA sequence result from environmental factors or are committed by DNA polymerases during replication. If unchecked, these errors might accumulate genetic damage such that the cell could no longer function. Thus, DNA repair processes involve mechanisms for the excision of damaged sequences and the resynthesis and ligation of the proper sequence. In mammalian cells, this proofreading function rests with DNA polymerase (pol) δ, a heterodimer of a 50kDa subunit, which stimulates pol δ activity in the presence of PCNA (proliferating cell nuclear antigen) and a 125kDa catalytic subunit. The catalytic subunit has 3' to 5' exonuclease activity which distinguishes pol δ from pol α and pol β. Pol δ is also central to DNA replication where it functions in leading strand synthesis at the replication fork. The catalytic subunit is phosphorylated by G1 cyclin-dependent kinase-cyclin complexes and, via its N-terminal 249 amino acids, interacts with cdk2. However, phosphorylation has little or no effect on the activity of pol δ. Thus, DNA polymerase ä is essential for DNA replication and is unique in its ability to replace damaged sequences through the process of DNA excision repair.
Development References (5)
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Sun Y, Jiang Y, Zhang P. Expression and characterization of the small subunit of human DNA polymerase delta. J Biol Chem. 1997; 272(20):13013-13018. (Biology). View Reference
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Wu SM, Zhang P, Zeng XR. Characterization of the p125 subunit of human DNA polymerase delta and its deletion mutants. Interaction with cyclin-dependent kinase-cyclins. J Biol Chem. 1998; 273(16):9561-9569. (Biology). View Reference
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Yang CL, Chang LS, Zhang P. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta. Nucleic Acids Res. 1992; 20(4):735-745. (Biology). View Reference
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Zeng XR, Jiang Y, Zhang SJ, Hao H, Lee MY. DNA polymerase delta is involved in the cellular response to UV damage in human cells. J Biol Chem. 1994; 269(19):13748-13751. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
