Skip to main content Skip to navigation
Purified Mouse Anti-DNA Polymerase δ
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human DNA Polymerase δ aa. 60-261
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
125 kDa
250 µg/ml
AB_398285
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610972 Rev. 1
Antibody Details
Down Arrow Up Arrow
22/DNA Polymerase δ

Errors in DNA sequence result from environmental factors or are committed by DNA polymerases during replication. If unchecked, these errors might accumulate genetic damage such that the cell could no longer function. Thus, DNA repair processes involve mechanisms for the excision of damaged sequences and the resynthesis and ligation of the proper sequence. In mammalian cells, this proofreading function rests with DNA polymerase (pol) δ, a heterodimer of a 50kDa subunit, which stimulates pol δ activity in the presence of PCNA (proliferating cell nuclear antigen) and a 125kDa catalytic subunit. The catalytic subunit has 3' to 5' exonuclease activity which distinguishes pol δ from pol α and pol β. Pol δ is also central to DNA replication where it functions in leading strand synthesis at the replication fork. The catalytic subunit is phosphorylated by G1 cyclin-dependent kinase-cyclin complexes and, via its N-terminal 249 amino acids, interacts with cdk2. However, phosphorylation has little or no effect on the  activity of pol δ. Thus, DNA polymerase ä is essential for DNA replication and is unique in its ability to replace damaged sequences through the process of DNA excision repair.

610972 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610972 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology). View Reference
  2. Sun Y, Jiang Y, Zhang P. Expression and characterization of the small subunit of human DNA polymerase delta. J Biol Chem. 1997; 272(20):13013-13018. (Biology). View Reference
  3. Wu SM, Zhang P, Zeng XR. Characterization of the p125 subunit of human DNA polymerase delta and its deletion mutants. Interaction with cyclin-dependent kinase-cyclins. J Biol Chem. 1998; 273(16):9561-9569. (Biology). View Reference
  4. Yang CL, Chang LS, Zhang P. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta. Nucleic Acids Res. 1992; 20(4):735-745. (Biology). View Reference
  5. Zeng XR, Jiang Y, Zhang SJ, Hao H, Lee MY. DNA polymerase delta is involved in the cellular response to UV damage in human cells. J Biol Chem. 1994; 269(19):13748-13751. (Biology). View Reference
View All (5) View Less
610972 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.