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FITC Mouse Anti-Human EEA1
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human EEA1 aa. 3-281
Immunofluorescence (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Not Recommended)
180 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612006 Rev. 1
Antibody Details
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Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly α-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine "finger" motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity.

This antibody is routinely tested by immunofluorescence microscopy. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612006 Rev. 1
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
612006 Rev.1
Citations & References
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Development References (1)

  1. Mu FT, Callaghan JM, Steele-Mortimer O, et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J Biol Chem. 1995; 270(22):13503-13511. (Biology). View Reference
612006 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.