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BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD28
Clone CD28.2 (RUO)
Flow cytometric analysis of CD28 expression by Human peripheral blood lymphocytes. Human whole blood was stained with Purified NA/LE Mouse Anti-Human CD28 antibody (Cat. No. 555725/567116/567117; solid line histogram). The cells were washed and then counterstained with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CD28 (or Ig Isotype control staining shown as dashed line histogram) was generated from gated events with the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD Flow Cytometer System.
Flow cytometric analysis of CD28 expression by Human peripheral blood lymphocytes. Human whole blood was stained with Purified NA/LE Mouse Anti-Human CD28 antibody (Cat. No. 555725/567116/567117; solid line histogram). The cells were washed and then counterstained with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CD28 (or Ig Isotype control staining shown as dashed line histogram) was generated from gated events with the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD Flow Cytometer System.
Flow cytometric analysis of CD28 expression by Human peripheral blood lymphocytes. Human whole blood was stained with Purified NA/LE Mouse Anti-Human CD28 antibody (Cat. No. 555725/567116/567117; solid line histogram). The cells were washed and then counterstained with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CD28 (or Ig Isotype control staining shown as dashed line histogram) was generated from gated events with the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD Flow Cytometer System.
Flow cytometric analysis of CD28 expression by Human peripheral blood lymphocytes. Human whole blood was stained with Purified NA/LE Mouse Anti-Human CD28 antibody (Cat. No. 555725/567116/567117; solid line histogram). The cells were washed and then counterstained with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CD28 (or Ig Isotype control staining shown as dashed line histogram) was generated from gated events with the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD Flow Cytometer System.
Product Details
BD Pharmingen™
CD28 antigen; T44; Tp44; TP44
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse C3H x BALB/c IgG1, κ
Human CD28 Transfected Cell Line
Flow cytometry (Routinely Tested), (Co)-stimulation (Tested During Development)
1.0 mg/ml
V 5T CD28.05
AB_396068
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2 µm sterile filtered. Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.
Recommended Assay Procedures
The CD28.2 monoclonal antibody is reportedly useful for multiple applications including the co-stimulated increase in cytokine production and proliferative responses by Anti-CD3 antibody-stimulated T cells.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- This product is sold under license. Purchase of this product does not include rights to (i) incorporate this product into the purchaser's own products for resale to end-users, or (ii) use this product to conduct for-profit research for or on behalf of another party. For information on obtaining a license to this product for such prohibited uses, contact INSERM, 7 rue Watt, 75013 Paris. Telephone: +33 1 55 03 01 60. Facsimile: +33 1 55 03 01 18. Email: techtransfert@inserm-transfert.fr
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
Purified NA/LE Mouse Anti-Human CD28 RUO
Size
2.0 mg
Cat No.
567117
555725 Rev. 17
Antibody Details
CD28.2
The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.
555725 Rev. 17
Format Details
NA/LE
Purified NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin. Purified NA/LE antibodies are well suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
555725 Rev.17
Citations & References
View product citations for antibody "555725" on CiteAb
Development References (10)
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Bjornson-Hooper ZB, Fragiadakis GK, Spitzer MH, et al. A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates.. Front Immunol. 2022; 13:867015. (Clone-specific: Flow cytometry). View Reference
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June CH, Bluestone JA, Nadler LM, Thompson CB. The B7 and CD28 receptor families. Immunol Today. 1994; 15(7):321-331. (Biology). View Reference
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Kuiper H, Brouwer M, Vermeire S, van Lier R. Analysis of the Workshop CD28 Panel mAb: distinct signalling pathways coupled to CD28. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:373-374.
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Lin G-X, Yang X, Hollemweguer E, et al. Cross-reactivity of CD antibodies in eight animal species. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:519-523.
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Nunes J, Klasen S, Franco MD, et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993; 293(3):835-842. (Clone-specific: Calcium Flux, (Co)-stimulation, Functional assay). View Reference
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Nunes J, Klasen S, Ragueneau M, et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993; 5(3):311-315. (Immunogen: Calcium Flux, (Co)-stimulation, Flow cytometry, Functional assay, IC/FCM Block, Immunoprecipitation, Stimulation). View Reference
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Olive D, Cerdan C, Costello R, et al. CD28 and CTLA-4 cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:360-370.
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Thaker YR, Schneider H, Rudd CE. TCR and CD28 activate the transcription factor NF-κB in T-cells via distinct adaptor signaling complexes.. Immunol Lett. 2015; 163(1):113-9. (Clone-specific: Activation, Flow cytometry). View Reference
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Tonsho M, Lee S, Aoyama A, et al. Tolerance of Lung Allografts Achieved in Nonhuman Primates via Mixed Hematopoietic Chimerism. Am J Transplant.. 2015; 15(8):2231-9. (Clone-specific: Flow cytometry). View Reference
555725 Rev. 17
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.