Purified NA/LE Hamster Anti-Mouse IL-9

BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse IL-9

Name: Purified NA/LE Hamster Anti-Mouse IL-9
Brand: BD Pharmingen™
SKU: 554472

Clone D9302C12

(RUO)

Product Details

Brand: BD Pharmingen™

Alternative Name: IL-9; Interleukin-9; MEA; P40; T-cell growth factor P40; TCGF III

Reactivity: Mouse (QC Testing)

Isotype: Armenian Hamster IgG2, κ

Immunogen: Mouse IL-9 Recombinant Protein

Application: ELISA (Routinely Tested), Neutralization (Tested During Development)

Concentration: 1.0 mg/ml

RRID: AB_395416

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

ELISA: Biotin Hamster Anti-Mouse IL-9 antibody (Clone D9302C12, Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels and can be paired with Purified Rat Anti-Mouse IL-9 (Clone D8402E8, Cat. No. 551218) as the capture antibody with recombinant mouse IL-9 as the standard.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Hamster IgG2, κ Isotype Control RUO

Size 0.5 mg Cat No. 559339

Biotin Hamster Anti-Mouse IL-9 RUO

Size 0.5 mg Cat No. 554473

Purified Rat Anti-Mouse IL-9 RUO

Size 1 mg Cat No. 551218

554472 Rev. 2

Antibody Details

D9302C12

The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Rα) and common gamma chain (γc) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.

554472 Rev. 2

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

554472 Rev.2

Citations & References

Development References (3)

Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
Nabel G, Galli SJ, Dvorak AM, Dvorak HF, Cantor H. Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells. Nature. 1981; 291(5813):332-334. (Biology). View Reference
Renauld JC, Kermouni A, Vink A, Louahed J, Van Snick J. Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis. J Leukoc Biol. 1995; 57(3):353-360. (Biology). View Reference

554472 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.