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Purified Mouse Anti-Rat IL-6
Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse IgG1, κ
Recombinant Rat IL-6
ELISA Capture (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified C3-4 antibody (Cat. No. 550644) is useful as a capture antibody for a sandwich ELISA for specifically measuring rat IL-6 protein levels. Purified C3-4 antibody can be paired with the biotinylated G307-2 antibody (Cat. No. 550642) as the detection antibody and with recombinant rat IL-6 protein (Cat. No. 557008) as the standard. The purified C3-4 capture antibody should be titrated between 2 - 8 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of rat IL-6 ranging from ~10,000 to 78 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site,w

Note 1: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring rat IL-6 in serum or plasma our rat IL-6 BD OptEIA™ ELISA Set (Cat. No. 550319) is especially formulated and recommended.

Note 2: This ELISA pair shows no cross-reactivity with rat IL-2, IL-4, IL-10, IFNγ, GM-CSF, TNF, mouse or human IL-6.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550644 Rev. 1
Antibody Details
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The C3-4 antibody reacts with rat interleukin-6 (IL-6). The immunogen used to  generate the C3-4 hybridoma was recombinant rat IL-6.

550644 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550644 Rev.1
Citations & References
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550644 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.