Preparation And Storage
Recommended Assay Procedures
ELISA Detection: The biotinylated B8-6 antibody (Cat. No. 555037) is useful as a detection antibody in a sandwich ELISA for measuring human MIG protein levels. The biotinylated B8-6 antibody can be paired with the purified monoclonal antibody B8-11 (Cat. No. 555038) with recombinant human MIG (Cat. No. 554636) as the standard. The biotinylated B8-6 antibody should be titrated to determine optimal concentration for ELISA detection (2.0 - 6.0 µg/ml). To obtain linear standard curves, doubling dilutions of human MIG ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
This ELISA pair shows no cross-reactivity with any of the cytokines tested ( human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, IP-10, lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TNF, LT-β).
This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum samples. The OptEIA™ Human MIG ELISA Set (Cat. No. 550998) is specially-formulated for serum cytokine measurement.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The monoclonal antibody B8-6 reacts with human monokine induced by gamma interferon (MIG). MIG is inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ, but not by IFN-α or bacterial lipopolysaccharides. The immunogen used to generate the monoclonal antibody B8-6 was insect cell-expressed, recombinant human MIG protein.
Development References (3)
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Farber JM. A macrophage mRNA selectively induced by gamma-interferon encodes a member of the platelet factor 4 family of cytokines. Proc Natl Acad Sci U S A. 1990; 87(14):5238-5242. (Biology). View Reference
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Farber JM. HuMig: a new human member of the chemokine family of cytokines. Biochem Biophys Res Commun. 1993; 192(1):223-230. (Biology). View Reference
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Liao F, Rabin RL, Yannelli JR, Koniaris LG, Vanguri P, Farber JM. Human Mig chemokine: biochemical and functional characterization. J Exp Med. 1995; 182(5):1301-1314. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.