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Biotin Mouse anti-Human IL-21
Biotin Mouse anti-Human IL-21

Human IL-21 sandwich ELISA curve: The curve was generated by a sandwich ELISA using the purified J148-1134 as capture antibody(Cat. No. 558503), doubling dilutions of recombinant human IL-21 protein and biotin I76-539 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214)were used to develop the ELISA.

Human IL-21 sandwich ELISA curve: The curve was generated by a sandwich ELISA using the purified J148-1134 as capture antibody(Cat. No. 558503), doubling dilutions of recombinant human IL-21 protein and biotin I76-539 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214)were used to develop the ELISA.

Product Details
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BD Pharmingen™
Mouse IgG1, κ
Human IL-21
ELISA (Routinely Tested)
0.5 mg/ml
AB_647172
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.

Recommended Assay Procedures

The biotinylated I76-539 mAb is useful as a detection antibody for a sandwich ELISA that measures human IL-21 protein when paired with purified mouse anti-human IL-21 (Cat. No. 558503) as capture antibody and recombinant human IL-21 protein as standard. The biotinylated I76-539 antibody should be titrated between 1 - 4 µg/ml to determine its optimal concentration. To obtain linear standard curves, doubling dilutions of recombinant human IL-21 protein ranging from 0.39 - 50 ng/ml are recommended. For specific methodology, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences.  For this ELISA antibody pair, no cross-reactivity has been observed with the following: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12/23p40, IL-12p70, IL-13, IL-15, IFN-γ, G-CSF, M-CSF, TNF and TGF-β.

Product Notices

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558502 Rev. 1
Antibody Details
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I76-539

The I76-539 antibody reacts with interleukin-21 (IL-21), an ~ 15 kDa member of the type-I-cytokine family of proteins. IL-21 is expressed primarily by CD4+ T cells and has a broad range of immunoregulatory functions on B cells, CD8+ T cells, NK cells and dendritic cells. The effects of IL-21 include promotion of antibody production, NK cell differentiation, proliferation and differentiation of cells of monocyte and granulocyte lineages, costimulation of T cells and augmentation of antitumor activity of CD8+ T cells. It has been speculated that IL-21, through its varies positive and negative regulatory effects on cell-mediated and humoral immunity, may play a role in autoimmuninty, allergy, asthma and tumor regression.

558502 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
558502 Rev.1
Citations & References
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Development References (2)

  1. Leonard WJ and Spolski R. Interleukin-21: A modulator of lymphoid proliferation, apoptosis and differentiation. Nat Rev Immunol. 2005; 5(9):688-698. (Biology). View Reference
  2. Sivakumar PV, Foster DC and Clegg CH. Interleukin-21 is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumour responses. Immunology. 2004; 112(2):177-182. (Biology). View Reference
558502 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.